Regression and persistence: Remodelling in a tissue engineered axial vascular assembly

Polykandriotis E, Euler S, Arkudas A, Pryymachuk G, Beier J, Greil P, Dragu A, Lametschwandtner A, Kneser U, Horch RE (2009)

Publication Language: English

Publication Type: Journal article

Publication year: 2009

Journal

Book Volume: 13

Pages Range: 4166-4175

Journal Issue: 10

DOI: 10.1111/j.1582-4934.2009.00828.x

Abstract

In later stages of vasculoangiogenesis a vascular network is going through a metamorphosis for optimal perfusion and economy of energy. In this study we make a quantitative approach to phenomena of remodelling in a bioartificial neovascular network and suggest variance of calibre as a parameter of neovascular maturation. For this study, 18 male Lewis rats were subjected to the AV loop operation in combination with a hard porous biogenic matrix and an isolation chamber. The animals were allocated into three groups for different explantation intervals set to 2, 4 and 8 weeks, respectively. Collective attributes like vascular density, percent fractional area and variance of calibre were evaluated for a predefined region of interest (ROI). Late morphogenesis was evaluated by means of scanning electron microscopy. After the fourth week the absolute number of vessels within the ROI decreased (P < 0.03) whereas, on the contrary, the fractional area of all segments increased (P < 0.02). The variance in calibre was significantly increased in the 8-week group (P < 0.05). Lymphatic growth after week 4, early pericyte migration as well as intussusceptive angiogenesis were identified immunohistologically. Phenomena of remodelling were evaluated quantitatively in a neovascular network and variance could be proposed as a parameter of net vascular maturation. © 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.

Authors with CRIS profile

Involved external institutions

Leopold-Franzens-Universität Innsbruck / University of Innsbruck Austria (AT) Universität Salzburg (Paris Lodron Universität Salzburg) Austria (AT)

How to cite

APA:

Polykandriotis, E., Euler, S., Arkudas, A., Pryymachuk, G., Beier, J., Greil, P.,... Horch, R.E. (2009). Regression and persistence: Remodelling in a tissue engineered axial vascular assembly. Journal of Cellular and Molecular Medicine, 13(10), 4166-4175. https://doi.org/10.1111/j.1582-4934.2009.00828.x

MLA:

Polykandriotis, Elias, et al. "Regression and persistence: Remodelling in a tissue engineered axial vascular assembly." Journal of Cellular and Molecular Medicine 13.10 (2009): 4166-4175.

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