Axial vascularization of a large volume calcium phosphate ceramic bone substitute in the sheep AV loop model

Beier J, Horch RE, Heß A, Arkudas A, Heinrich J, Loew J, Gulle H, Polykandriotis E, Bleiziffer O, Kneser U (2010)

Publication Language: English

Publication Type: Journal article

Publication year: 2010

Journal

Journal of Tissue Engineering and Regenerative Medicine Wiley-Blackwell

Book Volume: 4

Pages Range: 216-223

Journal Issue: 3

DOI: 10.1002/term.229

Abstract

Vascularization still remains an obstacle to engineering of bone tissue with clinically relevant dimensions. Our aim was to induce axial vascularization in a large volume of a clinically approved biphasic calcium phosphate ceramic by transferring the arteriovenous (AV) loop approach to a large animal model. HA/β-TCP granula were mixed with fibrin gel for a total volume of 16 cm3, followed by incorporation into an isolation chamber together with an AV loop. The chambers were implanted into the groins of merino sheep and the development of vascularization was monitored by sequential non-invasive magnetic resonance imaging (MRI). The chambers were explanted after 6 and 12 weeks, the pedicle was perfused with contrast agent and specimens were subjected to micro-computed tomography (μ-CT) scan and histological analysis. Sequential MRI demonstrated a significantly increased perfusion in the HA/β-TCP matrices over time. Micro-CT scans and histology confirmed successful axial vascularization of HA/β-TCP constructs. This study demonstrates, for the first time, successful axial vascularization of a clinically approved bone substitute with a significant volume in a large animal model by means of a microsurgically created AV loop, thus paving the way for the first microsurgical transplantation of a tissue-engineered, axially vascularized bone with clinically relevant dimensions. Copyright © 2009 John Wiley & Sons, Ltd.

Authors with CRIS profile

Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Andreas Heß Lehrstuhl für Pharmakologie und Toxikologie Andreas Arkudas Department of Plastic and Hand Surgery Johanna Heinrich Department of Plastic and Hand Surgery Elias Polykandriotis Department of Plastic and Hand Surgery

Involved external institutions

Baxter Aktiengesellschaft AT Austria (AT)

How to cite

APA:

Beier, J., Horch, R.E., Heß, A., Arkudas, A., Heinrich, J., Loew, J.,... Kneser, U. (2010). Axial vascularization of a large volume calcium phosphate ceramic bone substitute in the sheep AV loop model. Journal of Tissue Engineering and Regenerative Medicine, 4(3), 216-223. https://doi.org/10.1002/term.229

MLA:

Beier, Justus, et al. "Axial vascularization of a large volume calcium phosphate ceramic bone substitute in the sheep AV loop model." Journal of Tissue Engineering and Regenerative Medicine 4.3 (2010): 216-223.

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