De novo generation of an axially vascularized processed bovine cancellous-bone substitute in the sheep arteriovenous-loop model

Beier J, Heß A, Loew J, Heinrich J, Boos A, Arkudas A, Polykandriotis E, Bleiziffer O, Horch RE, Kneser U (2011)

Publication Type: Journal article

Publication year: 2011

Journal

European Surgical Research Karger

Book Volume: 46

Pages Range: 148-155

Journal Issue: 3

DOI: 10.1159/000324408

Abstract

Background/Aims: The aim of this study was to generate an axially vascularized bone substitute. The arteriovenous (AV)-loop approach in a large-animal model was applied in order to induce axial vascularization in a clinically approved processed bovine cancellous bone (PBCB) matrix of significant volume with primary mechanical stability and to assess the course of increasing axial vascularization. Methods: PBCB constructs were implanted into 13 merino sheep together with a microsurgically created AV loop in an isolation chamber. The vascularization process was monitored by sequential magnetic resonance imaging (MRI) scans. Explants were subjected to micro-computed tomography (micro-CT) analysis, histomorphometry and immunohistochemistry for CD31 and CD45. Results: Increasing axial vascularization in PBCB constructs was quantified by histomorphometry and visualized by micro-CT scans. Intravital sequential MRI scans demonstrated a significant progressive increase in perfused volume within the matrices. Immunohistochemistry confirmed endothelial lining of newly formed vessels. Conclusion: This study demonstrates successful axial vascularization of a clinically approved, mechanically stable bone substitute with a significant volume by a microsurgical AV loop in a large-animal model. Thus microsurgical transplantation of a tissue-engineered, axially vascularized and mechanically stable bone substitute with clinically relevant dimensions may become clinically feasible in the future. Copyright © 2011 S. Karger AG, Basel.

Authors with CRIS profile

Andreas Heß Lehrstuhl für Pharmakologie und Toxikologie Johanna Heinrich Medizinische Fakultät Anja Boos Department of Plastic and Hand Surgery Andreas Arkudas Department of Plastic and Hand Surgery Elias Polykandriotis Medizinische Fakultät Raymund Horch Professur für Plastische Chirurgie und Handchirurgie

How to cite

APA:

Beier, J., Heß, A., Loew, J., Heinrich, J., Boos, A., Arkudas, A.,... Kneser, U. (2011). De novo generation of an axially vascularized processed bovine cancellous-bone substitute in the sheep arteriovenous-loop model. European Surgical Research, 46(3), 148-155. https://doi.org/10.1159/000324408

MLA:

Beier, Justus, et al. "De novo generation of an axially vascularized processed bovine cancellous-bone substitute in the sheep arteriovenous-loop model." European Surgical Research 46.3 (2011): 148-155.

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