Involvement of TGF-β signaling pathway-associated genes in the corneal endothelium of patients with Fuchs endothelial corneal dystrophy
Nakagawa T, Honda T, Inagaki S, Yuasa T, Tourtas T, Schlötzer-Schrehardt U, Kruse F, Aouimeur I, Vaitinadapoule H, Travers G, He Z, Gain P, Koizumi N, Thuret G, Okumura N (2025)
Publication Type: Journal article
Publication year: 2025
Journal
Book Volume: 255
Article Number: 110334
DOI: 10.1016/j.exer.2025.110334
Abstract
This study investigated the involvement of TGF-β signaling pathway-associated genes in the pathogenesis of Fuchs endothelial corneal dystrophy (FECD). The RNA-sequencing analysis of corneal endothelial cells (CECs) from FECD patients revealed significant alterations in multiple TGF-β superfamily genes, with 9 genes upregulated (including BMP6, GDF5, and TGF-β2) and 10 genes downregulated (including BMP2, NOG, and INHBA) compared to controls. Quantitative PCR validation confirmed the elevated expression of GDF5 (3.35-fold in non-expanded and 7.66-fold in expanded TCF4), TGF-β2 (6.17-fold and 11.5-fold), and TGF-β1 (1.78-fold and 1.58-fold) in FECD patients with and without TCF4 trinucleotide repeat expansion. Ex-vivo experiments using donor corneas demonstrated that TGF-β2 stimulation significantly increased the expression of extracellular matrix (ECM) components associated with guttae formation, including fibronectin, types I and VI collagens, and other matrix proteins. Immunofluorescence confirmed increased fibronectin protein expression in the corneal endothelium following TGF-β1 or TGF-β2 treatment. This study provides the first comprehensive analysis of TGF-β superfamily involvement in FECD and suggests that GDF5, found to be upregulated in FECD, may contribute to the disease process. These findings further indicate that dysregulation of TGF-β signaling pathways drives the characteristic ECM accumulation in FECD, potentially offering new therapeutic targets for this progressive corneal disease involving fibrosis-related alterations. Future research is warranted to clarify GDF5's specific role and mechanistic impact on FECD pathogenesis.
Authors with CRIS profile
Theofilos Tourtas
Lehrstuhl für Augenheilkunde
Ursula Schlötzer-Schrehardt
Medizinische Fakultät
Friedrich Kruse
Lehrstuhl für Augenheilkunde
Involved external institutions
Japan (JP)
France (FR)How to cite
APA:
Nakagawa, T., Honda, T., Inagaki, S., Yuasa, T., Tourtas, T., Schlötzer-Schrehardt, U.,... Okumura, N. (2025). Involvement of TGF-β signaling pathway-associated genes in the corneal endothelium of patients with Fuchs endothelial corneal dystrophy. Experimental Eye Research, 255. https://doi.org/10.1016/j.exer.2025.110334
MLA:
Nakagawa, Tatsuya, et al. "Involvement of TGF-β signaling pathway-associated genes in the corneal endothelium of patients with Fuchs endothelial corneal dystrophy." Experimental Eye Research 255 (2025).
BibTeX: Download