Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC

Dudziak D, Heger L, Agace WW, Bakker J, de Gruijl TD, Dress RJ, Dutertre CA, Fenton TM, Fransen MF, Ginhoux F, Heyman O, Horev Y, Hornsteiner F, Kandiah V, Kles P, Lubin R, Mizraji G, Prokopi A, Saar O, Sopper S, Stoitzner P, Strandt H, Sykora MM, Toffoli EC, Tripp CH, van Pul K, van de Ven R, Wilensky A, Yona S, Zelle-Rieser C (2025)

Publication Type: Journal article

Publication year: 2025

Journal

European Journal of Immunology Wiley-VCH Verlag

Book Volume: 55

Article Number: 2250325

Journal Issue: 1

DOI: 10.1002/eji.202250325

Abstract

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.

Authors with CRIS profile

Lukas Heger Department of Dermatology

Involved external institutions

Universitätsklinikum Jena DE Germany (DE) University of Copenhagen DK Denmark (DK) Amsterdam institute for Immunology and Infectious Diseases NL Netherlands (NL) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Hebrew University of Jerusalem IL Israel (IL) Immunologie intégrative des tumeurs et immunothérapie du cancer FR France (FR) University of Glasgow GB United Kingdom (GB) Singapore Immunology Network (SIgN) SG Singapore (SG) Medizinische Universität Innsbruck AT Austria (AT) Amsterdam University Medical Centers (Amsterdam UMC) / Amsterdam Universitair Medische Centra NL Netherlands (NL)

How to cite

APA:

Dudziak, D., Heger, L., Agace, W.W., Bakker, J., de Gruijl, T.D., Dress, R.J.,... Zelle-Rieser, C. (2025). Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC. European Journal of Immunology, 55(1). https://doi.org/10.1002/eji.202250325

MLA:

Dudziak, Diana, et al. "Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC." European Journal of Immunology 55.1 (2025).

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