Heger L, Amon L, Lehmann C, Dudziak D (2020)
Publication Type: Authored book
Publication year: 2020
Publisher: Elsevier
ISBN: 9780128160770
DOI: 10.1016/B978-0-12-801238-3.11609-5
Since their discovery in the 1970s, Dendritic cells (DCs) emerged as central regulators of immune responses controlling the induction and type of T cell immunity. There, DCs orchestrate the delicate balance of inducing protective immune responses against pathogens and tumors, while maintaining tolerance to self. Owing to their low abundance, short life, sensitivity and diversity, researchers had a hard time to dissect and identify specialized DC subsets in mice and men, track their ontogenetic origin and characterize key phenotypic and functional features utilizable for future therapeutic approaches. Thus, the DC community had to empower every technical innovation over the last decades as soon as possible. These advances range from the development of isolation and enrichment protocols to the generation of DC specific antibodies, early subtraction cDNA library screenings, bulk RNA sequencing and microarrays, simple flow cytometry and cell sorting to multicolor flow- and mass cytometry and unbiased -omics studies including analysis of the epigenome, transcriptome, lipidome and proteome. Within this review, we aim to briefly summarize the history of dendritic cell research ranging from their discovery to the onset of cutting-edge techniques with focus on unbiased single cell based approaches currently revolutionizing our understanding of DC biology.
APA:
Heger, L., Amon, L., Lehmann, C., & Dudziak, D. (2020). Systems Immunology Approaches for Understanding of Primary Dendritic Cell Subpopulations in the Past, Present and Future. Elsevier.
MLA:
Heger, Lukas, et al. Systems Immunology Approaches for Understanding of Primary Dendritic Cell Subpopulations in the Past, Present and Future. Elsevier, 2020.
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