Analysis of abiotic and biotic stress-induced Ca2+ transients in the crop species Solanum tuberosum

van Dieren A, Schwarzenbacher RE, Sonnewald S, Bittner A, Vothknecht UC (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 14

Article Number: 27625

Journal Issue: 1

DOI: 10.1038/s41598-024-79134-3

Abstract

Secondary messengers, such as calcium ions (Ca2+), are integral parts of a system that transduces environmental stimuli into appropriate cellular responses. Different abiotic and biotic stresses as well as developmental processes trigger temporal increases in cytosolic free Ca2+ levels by an influx from external and internal stores. Stimulus-specificity is obtained by a certain amplitude, duration, oscillation and localisation of the response. Most knowledge on stress-specific Ca2+ transient, called calcium signatures, has been gained in the model plant Arabidopsis thaliana, while reports about stress-related Ca2+ signalling in crop plants are comparatively scarce. In this study, we introduced the Ca2+ biosensor apoaequorin into potato (Solanum tuberosum, Lcv. Désirée). We observed dose-dependent calcium signatures in response to a series of stress stimuli, including H2O2, NaCl, mannitol and pathogen-associated molecular patterns (PAMPs) with stimuli-specific kinetics. Direct comparison with Arabidopsis revealed differences in the kinetics and amplitude of Ca2+ transients between both species, implying species-specific sensitivity for different stress conditions. The potato line generated in this work provides a useful tool for further investigations on stress-induced signalling pathways, which could contribute to the generation of novel, stress-tolerant potato varieties.

Authors with CRIS profile

Sophia Sonnewald Department Biologie

Involved external institutions

Durham University GB United Kingdom (GB) Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE)

How to cite

APA:

van Dieren, A., Schwarzenbacher, R.E., Sonnewald, S., Bittner, A., & Vothknecht, U.C. (2024). Analysis of abiotic and biotic stress-induced Ca2+ transients in the crop species Solanum tuberosum. Scientific Reports, 14(1). https://doi.org/10.1038/s41598-024-79134-3

MLA:

van Dieren, Annelotte, et al. "Analysis of abiotic and biotic stress-induced Ca2+ transients in the crop species Solanum tuberosum." Scientific Reports 14.1 (2024).

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