Di Berardino C, Peserico A, Camerano Spelta Rapini C, Liverani L, Capacchietti G, Russo V, Berardinelli P, Ünalan I, Damian-Buda AI, Boccaccini AR, Barboni B (2024)
Publication Type: Journal article
Publication year: 2024
Book Volume: 22
Article Number: 95
Journal Issue: 1
DOI: 10.1186/s12958-024-01266-y
Background: Assisted Reproductive Technologies (ARTs) have been validated in human and animal to solve reproductive problems such as infertility, aging, genetic selection/amplification and diseases. The persistent gap in ART biomedical applications lies in recapitulating the early stage of ovarian folliculogenesis, thus providing protocols to drive the large reserve of immature follicles towards the gonadotropin-dependent phase. Tissue engineering is becoming a concrete solution to potentially recapitulate ovarian structure, mostly relying on the use of autologous early follicles on natural or synthetic scaffolds. Based on these premises, the present study has been designed to validate the use of the ovarian bioinspired patterned electrospun fibrous scaffolds fabricated with poly(ε-caprolactone) (PCL) for multiple preantral (PA) follicle development. Methods: PA follicles isolated from lamb ovaries were cultured on PCL scaffold adopting a validated single-follicle protocol (Ctrl) or simulating a multiple-follicle condition by reproducing an artificial ovary engrafted with 5 or 10 PA (AO
APA:
Di Berardino, C., Peserico, A., Camerano Spelta Rapini, C., Liverani, L., Capacchietti, G., Russo, V.,... Barboni, B. (2024). Bioengineered 3D ovarian model for long-term multiple development of preantral follicle: bridging the gap for poly(ε-caprolactone) (PCL)-based scaffold reproductive applications. Reproductive Biology and Endocrinology, 22(1). https://doi.org/10.1186/s12958-024-01266-y
MLA:
Di Berardino, Chiara, et al. "Bioengineered 3D ovarian model for long-term multiple development of preantral follicle: bridging the gap for poly(ε-caprolactone) (PCL)-based scaffold reproductive applications." Reproductive Biology and Endocrinology 22.1 (2024).
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