Incorporation of montmorillonite into microfluidics-generated chitosan microfibers enhances neuron-like PC12 cells for application in neural tissue engineering

Katoli Z, Navaei-Nigjeh M, Mirzababaei S, Sabahi H, Baeeri M, Akrami M, Roshanbinfar K, Engel F, Abdollahi M (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Carbohydrate Polymers Elsevier

Book Volume: 342

Article Number: 122272

DOI: 10.1016/j.carbpol.2024.122272

Abstract

The complexity in structure and function of the nervous system, as well as its slow rate of regeneration, makes it more difficult to treat it compared to other tissues. Neural tissue engineering aims to create an appropriate environment for nerve cell proliferation and differentiation. Fibrous scaffolds with suitable morphology and topography and better mimicry of the extracellular matrix have been promising for the alignment and migration of neural cells. On this premise, to improve the properties of the scaffold, we combined montmorillonite (MMT) with chitosan (CS) polymer and created microfibers with variable diameters and varied concentrations of MMT using microfluidic technology and tested its suitability for the rat pheochromocytoma cell line (PC12). According to the findings, CS/MMT 0.1 % compared to CS/MMT 0 % microfibers showed a 201 MPa increase in Young's modulus, a 68 mS/m increase in conductivity, and a 1.4-fold increase in output voltage. Analysis of cell mitochondrial activity verified the non-toxicity, resulting in good cell morphology with orientation along the microfiber. Overall, the results of this project showed that with a low concentration of MMT, the properties of microfibers can be significantly improved and a suitable scaffold can be designed for neural tissue engineering.

Authors with CRIS profile

Kaveh Roshanbinfar Professur für Experimentelle Nieren- und Kreislaufforschung Felix Engel Professur für Experimentelle Nieren- und Kreislaufforschung

Involved external institutions

University of Tehran IR Iran, Islamic Republic of (IR) Tehran University of Medical Sciences (TUMS) / دانشگاه علوم پزشکی تهران IR Iran, Islamic Republic of (IR)

How to cite

APA:

Katoli, Z., Navaei-Nigjeh, M., Mirzababaei, S., Sabahi, H., Baeeri, M., Akrami, M.,... Abdollahi, M. (2024). Incorporation of montmorillonite into microfluidics-generated chitosan microfibers enhances neuron-like PC12 cells for application in neural tissue engineering. Carbohydrate Polymers, 342. https://doi.org/10.1016/j.carbpol.2024.122272

MLA:

Katoli, Zahra, et al. "Incorporation of montmorillonite into microfluidics-generated chitosan microfibers enhances neuron-like PC12 cells for application in neural tissue engineering." Carbohydrate Polymers 342 (2024).

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