Tensorial tomographic Fourier ptychography with applications to muscle tissue imaging

Xu S, Yang X, Ritter P, Dai X, Lee KC, Kreiß L, Zhou KC, Kim K, Chaware A, Neff J, Glass C, Lee SA, Friedrich O, Horstmeyer R (2024)

Publication Language: English

Publication Type: Journal article

Publication year: 2024

Journal

Advanced Photonics SPIE

Book Volume: 6

Article Number: 026004

Journal Issue: 2

DOI: 10.1117/1.AP.6.2.026004

Abstract

We report tensorial tomographic Fourier ptychography (T2oFu), a nonscanning label-free tomographic microscopy method for simultaneous imaging of quantitative phase and anisotropic specimen information in 3D. Built upon Fourier ptychography, a quantitative phase imaging technique, T2oFu additionally highlights the vectorial nature of light. The imaging setup consists of a standard microscope equipped with an LED matrix, a polarization generator, and a polarization-sensitive camera. Permittivity tensors of anisotropic samples are computationally recovered from polarized intensity measurements across three dimensions. We demonstrate T2oFu’s efficiency through volumetric reconstructions of refractive index, birefringence, and orientation for various validation samples, as well as tissue samples from muscle fibers and diseased heart tissue. Our reconstructions of healthy muscle fibers reveal their 3D fine-filament structures with consistent orientations. Additionally, we demonstrate reconstructions of a heart tissue sample that carries important polarization information for detecting cardiac amyloidosis.

Authors with CRIS profile

Paul Ritter Lehrstuhl für Medizinische Biotechnologie Lucas Kreiß Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

Yonsei University KR Korea, Republic of (KR) Duke University US United States (USA) (US) Duke University Medical Center US United States (USA) (US)

How to cite

APA:

Xu, S., Yang, X., Ritter, P., Dai, X., Lee, K.C., Kreiß, L.,... Horstmeyer, R. (2024). Tensorial tomographic Fourier ptychography with applications to muscle tissue imaging. Advanced Photonics, 6(2). https://doi.org/10.1117/1.AP.6.2.026004

MLA:

Xu, Shiqi, et al. "Tensorial tomographic Fourier ptychography with applications to muscle tissue imaging." Advanced Photonics 6.2 (2024).

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