Comparison of Extracellular Vesicles from Induced Pluripotent Stem Cell-Derived Brain Cells
Xavier G, Navarrete Santos A, Hartmann C, Santoro ML, Flegel N, Reinsch J, Majer A, Ehrhardt T, Pfeifer J, Simm A, Hollemann T, Belangero SI, Rujescu D, Jung M (2024)
Publication Type: Journal article
Publication year: 2024
Journal
Book Volume: 25
Article Number: 3575
Journal Issue: 7
DOI: 10.3390/ijms25073575
Abstract
The pathophysiology of many neuropsychiatric disorders is still poorly understood. Identification of biomarkers for these diseases could benefit patients due to better classification and stratification. Exosomes excreted into the circulatory system can cross the blood–brain barrier and carry a cell type-specific set of molecules. Thus, exosomes are a source of potential biomarkers for many diseases, including neuropsychiatric disorders. Here, we investigated exosomal proteins produced from human-induced pluripotent stem cells (iPSCs) and iPSC-derived neural stem cells, neural progenitors, neurons, astrocytes, microglia-like cells, and brain capillary endothelial cells. Of the 31 exosome surface markers analyzed, a subset of biomarkers were significantly enriched in astrocytes (CD29, CD44, and CD49e), microglia-like cells (CD44), and neural stem cells (SSEA4). To identify molecular fingerprints associated with disease, circulating exosomes derived from healthy control (HC) individuals were compared against schizophrenia (SCZ) patients and late-onset Alzheimer’s disease (LOAD) patients. A significant epitope pattern was identified for LOAD (CD1c and CD2) but not for SCZ compared to HC. Thus, analysis of cell type- and disease-specific exosome signatures of iPSC-derived cell cultures may provide a valuable model system to explore proteomic biomarkers for the identification of novel disease profiles.
Authors with CRIS profile
Involved external institutions
Federal University of São Paulo / Universidade Federal de São Paulo (UNIFESP)
Brazil (BR)
Universitätsklinikum Halle (Saale)
Germany (DE)
Martin-Luther-Universität Halle-Wittenberg (MLU)
Germany (DE)
Medizinische Universität Wien
Austria (AT)
Brazil (BR)
Universitätsklinikum Halle (Saale)
Germany (DE)
Martin-Luther-Universität Halle-Wittenberg (MLU)
Germany (DE)
Medizinische Universität Wien
Austria (AT)
How to cite
APA:
Xavier, G., Navarrete Santos, A., Hartmann, C., Santoro, M.L., Flegel, N., Reinsch, J.,... Jung, M. (2024). Comparison of Extracellular Vesicles from Induced Pluripotent Stem Cell-Derived Brain Cells. International Journal of Molecular Sciences, 25(7). https://doi.org/10.3390/ijms25073575
MLA:
Xavier, Gabriela, et al. "Comparison of Extracellular Vesicles from Induced Pluripotent Stem Cell-Derived Brain Cells." International Journal of Molecular Sciences 25.7 (2024).
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