Static magnetic field enhances the bone remodelling capacity of human demineralized bone matrix in a rat animal model of cranial bone defects

Hosseini SJ, Parsaei H, Moosavifar MJ, Tavakoli N, Ahadi R, Roshanbinfar K (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Journal of Materials Chemistry B Royal Society of Chemistry

Book Volume: 12

Pages Range: 3774-3785

Journal Issue: 15

DOI: 10.1039/d3tb02299d

Abstract

The regeneration of bone defects that exceed 2 cm is a challenge for the human body, necessitating interventional therapies. Demineralized bone matrices (DBM) derived from biological tissues have been employed for bone regeneration and possess notable osteoinductive and osteoconductive characteristics. Nevertheless, their efficiency in regenerating critically sized injuries is limited, and therefore additional signaling cues are required. Thanks to the piezoelectric properties of the bone, external physical stimulation is shown to accelerate tissue healing. We have implanted human DBM in critically sized cranial bone defects in rat animal models and exposed them to an external magnetic field (1 T) to enhance endogenous bone formation. Our in vitro experiments showed the superior cytocompatibility of DBM compared to cell culture plates. Furthermore, alkaline phosphatase activity after 14 days and Alizarin red staining at 28 days demonstrated differentiation of rat bone marrow mesenchymal stem cells into bone lineage on DBM. Computer tomography images together with histological analyses showed that implanting DBM in the injured rats significantly enhanced bone regeneration. Notably, combining DBM transplantation with a 2 h daily exposure to a 1 T magnetic field for 2 weeks (day 7 to 21 post-surgery) significantly improved bone regeneration compared to DBM transplantation alone. This research indicates that utilizing external magnetic stimulation significantly enhances the potential of bone allografts to regenerate critically sized bone defects.

Authors with CRIS profile

Kaveh Roshanbinfar Professur für Experimentelle Nieren- und Kreislaufforschung

Involved external institutions

Amirkabir University of Technology (AUT) / دانشگاه صنعتی امیرکبیر IR Iran, Islamic Republic of (IR) Semnan University of Medical Sciences / دانشگاه علوم پزشکی و خدمات بهداشتی درمانی سمنان‎ IR Iran, Islamic Republic of (IR) University of Tehran IR Iran, Islamic Republic of (IR) Iran University of Medical Sciences (IUMS) / دانشگاه علوم پزشکی ایران IR Iran, Islamic Republic of (IR)

How to cite

APA:

Hosseini, S.J., Parsaei, H., Moosavifar, M.J., Tavakoli, N., Ahadi, R., & Roshanbinfar, K. (2024). Static magnetic field enhances the bone remodelling capacity of human demineralized bone matrix in a rat animal model of cranial bone defects. Journal of Materials Chemistry B, 12(15), 3774-3785. https://doi.org/10.1039/d3tb02299d

MLA:

Hosseini, Seyed Jamal, et al. "Static magnetic field enhances the bone remodelling capacity of human demineralized bone matrix in a rat animal model of cranial bone defects." Journal of Materials Chemistry B 12.15 (2024): 3774-3785.

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