Discovery of a Novel Antiviral Effect of the Restriction Factor SPOC1 against Human Cytomegalovirus

Kuderna AK, Reichel A, Tillmanns J, Class M, Scherer M, Stamminger T (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Viruses MDPI

Book Volume: 16

Article Number: 363

Journal Issue: 3

DOI: 10.3390/v16030363

Abstract

The chromatin-remodeler SPOC1 (PHF13) is a transcriptional co-regulator and has been identified as a restriction factor against various viruses, including human cytomegalovirus (HCMV). For HCMV, SPOC1 was shown to block the onset of immediate-early (IE) gene expression under low multiplicities of infection (MOI). Here, we demonstrate that SPOC1-mediated restriction of IE expression is neutralized by increasing viral titers. Interestingly, our study reveals that SPOC1 exerts an additional antiviral function beyond the IE phase of HCMV replication. Expression of SPOC1 under conditions of high MOI resulted in severely impaired viral DNA replication and viral particle release, which may be attributed to inefficient viral transcription. With the use of click chemistry, the localization of viral DNA was investigated at late time points after infection. Intriguingly, we detected a co-localization of SPOC1, RNA polymerase II S5P and polycomb repressor complex 2 (PRC2) components in close proximity to viral DNA in areas that are hypothesized to harbor viral transcription sites. We further identified the N-terminal domain of SPOC1 to be responsible for interaction with EZH2, a subunit of the PRC2 complex. With this study, we report a novel and potent antiviral function of SPOC1 against HCMV that is efficient even with unrestricted IE gene expression.

Authors with CRIS profile

Julia Tillmanns Institute of Clinical and Molecular Virology

Involved external institutions

Universitätsklinikum Ulm DE Germany (DE) Instituto de Medicina Molecular (IMM) PT Portugal (PT)

How to cite

APA:

Kuderna, A.K., Reichel, A., Tillmanns, J., Class, M., Scherer, M., & Stamminger, T. (2024). Discovery of a Novel Antiviral Effect of the Restriction Factor SPOC1 against Human Cytomegalovirus. Viruses, 16(3). https://dx.doi.org/10.3390/v16030363

MLA:

Kuderna, Anna K., et al. "Discovery of a Novel Antiviral Effect of the Restriction Factor SPOC1 against Human Cytomegalovirus." Viruses 16.3 (2024).

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