An optofluidic antenna for enhancing the sensitivity of single-emitter measurements
Morales-Inostroza L, Folz J, Kühnemuth R, Felekyan S, Wieser FF, Seidel CA, Götzinger S, Sandoghdar V (2024)
Publication Type: Journal article
Publication year: 2024
Journal
Book Volume: 15
Article Number: 2545
Journal Issue: 1
DOI: 10.1038/s41467-024-46730-w
Abstract
Many single-molecule investigations are performed in fluidic environments, for example, to avoid unwanted consequences of contact with surfaces. Diffusion of molecules in this arrangement limits the observation time and the number of collected photons, thus, compromising studies of processes with fast or slow dynamics. Here, we introduce a planar optofluidic antenna (OFA), which enhances the fluorescence signal from molecules by about 5 times per passage, leads to about 7-fold more frequent returns to the observation volume, and significantly lengthens the diffusion time within one passage. We use single-molecule multi-parameter fluorescence detection (sm-MFD), fluorescence correlation spectroscopy (FCS) and Förster resonance energy transfer (FRET) measurements to characterize our OFAs. The antenna advantages are showcased by examining both the slow (ms) and fast (50 μs) dynamics of DNA four-way (Holliday) junctions with real-time resolution. The FRET trajectories provide evidence for the absence of an intermediate conformational state and introduce an upper bound for its lifetime. The ease of implementation and compatibility with various microscopy modalities make OFAs broadly applicable to a diverse range of studies.
Authors with CRIS profile
Additional Organisation(s)
Involved external institutions
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
Heinrich-Heine-Universität Düsseldorf
Germany (DE)
Germany (DE)
Heinrich-Heine-Universität Düsseldorf
Germany (DE)
How to cite
APA:
Morales-Inostroza, L., Folz, J., Kühnemuth, R., Felekyan, S., Wieser, F.F., Seidel, C.A.,... Sandoghdar, V. (2024). An optofluidic antenna for enhancing the sensitivity of single-emitter measurements. Nature Communications, 15(1). https://doi.org/10.1038/s41467-024-46730-w
MLA:
Morales-Inostroza, Luis, et al. "An optofluidic antenna for enhancing the sensitivity of single-emitter measurements." Nature Communications 15.1 (2024).
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