Lyman KA, Han Y, Robinson AP, Weinberg SE, Fisher DW, Heuermann RJ, Lyman RE, Kim DK, Ludwig A, Chandel NS, Does MD, Miller SD, Chetkovich DM (2024)
Publication Type: Journal article
Publication year: 2024
Book Volume: 18
DOI: 10.3389/fncel.2024.1321682
Mature oligodendrocytes (OLG) are the myelin-forming cells of the central nervous system. Recent work has shown a dynamic role for these cells in the plasticity of neural circuits, leading to a renewed interest in voltage-sensitive currents in OLG. Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and their respective current (Ih) were recently identified in mature OLG and shown to play a role in regulating myelin length. Here we provide a biochemical and electrophysiological characterization of HCN channels in cells of the oligodendrocyte lineage. We observed that mice with a nonsense mutation in the Hcn2 gene (Hcn2ap/ap) have less white matter than their wild type counterparts with fewer OLG and fewer oligodendrocyte progenitor cells (OPCs). Hcn2ap/ap mice have severe motor impairments, although these deficits were not observed in mice with HCN2 conditionally eliminated only in oligodendrocytes (Cnpcre/+; Hcn2F/F). However, Cnpcre/+; Hcn2F/F mice develop motor impairments more rapidly in response to experimental autoimmune encephalomyelitis (EAE). We conclude that HCN2 channels in OLG may play a role in regulating metabolism.
APA:
Lyman, K.A., Han, Y., Robinson, A.P., Weinberg, S.E., Fisher, D.W., Heuermann, R.J.,... Chetkovich, D.M. (2024). Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes. Frontiers in Cellular Neuroscience, 18. https://doi.org/10.3389/fncel.2024.1321682
MLA:
Lyman, Kyle A., et al. "Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes." Frontiers in Cellular Neuroscience 18 (2024).
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