Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes.

Lyman KA, Han Y, Robinson AP, Weinberg SE, Fisher DW, Heuermann RJ, Lyman RE, Kim DK, Ludwig A, Chandel NS, Does MD, Miller SD, Chetkovich DM (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Frontiers in Cellular Neuroscience Frontiers Research Foundation / Frontiers Media

Book Volume: 18

DOI: 10.3389/fncel.2024.1321682

Abstract

Mature oligodendrocytes (OLG) are the myelin-forming cells of the central nervous system. Recent work has shown a dynamic role for these cells in the plasticity of neural circuits, leading to a renewed interest in voltage-sensitive currents in OLG. Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and their respective current (Ih) were recently identified in mature OLG and shown to play a role in regulating myelin length. Here we provide a biochemical and electrophysiological characterization of HCN channels in cells of the oligodendrocyte lineage. We observed that mice with a nonsense mutation in the Hcn2 gene (Hcn2ap/ap) have less white matter than their wild type counterparts with fewer OLG and fewer oligodendrocyte progenitor cells (OPCs). Hcn2ap/ap mice have severe motor impairments, although these deficits were not observed in mice with HCN2 conditionally eliminated only in oligodendrocytes (Cnpcre/+; Hcn2F/F). However, Cnpcre/+; Hcn2F/F mice develop motor impairments more rapidly in response to experimental autoimmune encephalomyelitis (EAE). We conclude that HCN2 channels in OLG may play a role in regulating metabolism.

Authors with CRIS profile

Andreas Ludwig Lehrstuhl für Pharmakologie und Toxikologie

Involved external institutions

Northwestern University US United States (USA) (US) Vanderbilt University US United States (USA) (US) Washington University US United States (USA) (US) Ohio University US United States (USA) (US) University of Washington US United States (USA) (US) Massachusetts General Hospital US United States (USA) (US)

How to cite

APA:

Lyman, K.A., Han, Y., Robinson, A.P., Weinberg, S.E., Fisher, D.W., Heuermann, R.J.,... Chetkovich, D.M. (2024). Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes. Frontiers in Cellular Neuroscience, 18. https://dx.doi.org/10.3389/fncel.2024.1321682

MLA:

Lyman, Kyle A., et al. "Characterization of hyperpolarization-activated cyclic nucleotide-gated channels in oligodendrocytes." Frontiers in Cellular Neuroscience 18 (2024).

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