Actomyosin bundles serve as a tension sensor and a platform for ERK activation
Hirata H, Gupta M, Vedula SRK, Lim CT, Ladoux B, Sokabe M (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 16
Pages Range: 250-257
Journal Issue: 2
Abstract
Tensile forces generated by stress fibers drive signal transduction events at focal adhesions. Here, we report that stress fibers per se act as a platform for tension-induced activation of biochemical signals. The MAP kinase, ERK is activated on stress fibers in a myosin II-dependent manner. In myosin II-inhibited cells, uniaxial stretching of cell adhesion substrates restores ERK activation on stress fibers. By quantifying myosin II- or mechanical stretch-mediated tensile forces in individual stress fibers, we show that ERK activation on stress fibers correlates positively with tensile forces acting on the fibers, indicating stress fibers as a tension sensor in ERK activation. Myosin II-dependent ERK activation is also observed on actomyosin bundles connecting E-cadherin clusters, thus suggesting that actomyosin bundles, in general, work as a platform for tension-dependent ERK activation. Synopsis This study reveals that individual stress fibers work as a platform for activating ERK in a mechanical tension-dependent manner. Activation of ERK on stress fibers, but not its localization, depends on myosin II activity. External application of tensile force to stress fibers restores ERK activation on stress fibers under inhibition of myosin II. ERK activation on individual stress fibers increases with the magnitude of tensile forces acting on the fibers. This study reveals that individual stress fibers work as a platform for activating ERK in a mechanical tension-dependent manner.
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APA:
Hirata, H., Gupta, M., Vedula, S.R.K., Lim, C.T., Ladoux, B., & Sokabe, M. (2015). Actomyosin bundles serve as a tension sensor and a platform for ERK activation. EMBO Reports, 16(2), 250-257. https://doi.org/10.15252/embr.201439140
MLA:
Hirata, Hiroaki, et al. "Actomyosin bundles serve as a tension sensor and a platform for ERK activation." EMBO Reports 16.2 (2015): 250-257.
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