A Two-Stage Cascade for Increased High-Value Product Accumulation in Chlamydomonas asymmetrica

Hahm J, Jung SH, Kandaswamy S, Talwar P, Verma N, Vivekanand V, Lindenberger C (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Fermentation MDPI

Book Volume: 10

Article Number: 38

Journal Issue: 1

DOI: 10.3390/fermentation10010038

Abstract

Cascade systems are used in the large-scale production of astaxanthin, facilitating a successful value-added process despite high accumulating costs. However, their application to other high-value products (HVPs), like lutein, β-carotene, chlorophylls, and fatty acids, remains unexplored. This study investigates Chlamydomonas asymmetica in chemostatic cultures, focusing on the impact of light and dilution rate. A two-stage cascade system is designed, combining high-light growth with low-light pigment accumulation. The results show potential for productivity improvement. Notably, the spacetime yield (STY) of Chlorophyll a increased by 20.96%, reaching 2.73 g·L−1·d−1 at the lowest dilution rate. Lutein maintains a consistent concentration of 22.34 mg·g−1, while β-carotene achieves a maximum STY of 3.60 mg·L·d−1. A cascade modification with a hollow fiber membrane significantly enhances HVP concentrations—Chlorophyll b, Lutein, Chlorophyll a, β-carotene, EPS, and GLA increase 27.23%, 38.95%, 31.88%, 86.19%, 128.7%, and 57.71%, respectively. STY improvements for these HVPs range from 1.78% to 82.96%. This study offers insights into C. asymmetica’s response and proposes a cascade modification for enhanced HVP production and downstream processing efficiency.

Authors with CRIS profile

Joachim Hahm Technische Fakultät Christoph Lindenberger Department Chemie- und Bioingenieurwesen

Involved external institutions

Ostbayerische Technische Hochschule (OTH) Amberg-Weiden DE Germany (DE) Malaviya National Institute of Technology Jaipur (MNIT) / मालवीय राष्ट्रीय प्रौद्योगिकी संस्थान जयपुर IN India (IN)

How to cite

APA:

Hahm, J., Jung, S.H., Kandaswamy, S., Talwar, P., Verma, N., Vivekanand, V., & Lindenberger, C. (2024). A Two-Stage Cascade for Increased High-Value Product Accumulation in Chlamydomonas asymmetrica. Fermentation, 10(1). https://dx.doi.org/10.3390/fermentation10010038

MLA:

Hahm, Joachim, et al. "A Two-Stage Cascade for Increased High-Value Product Accumulation in Chlamydomonas asymmetrica." Fermentation 10.1 (2024).

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