Impact of CRISPR/Cas9-Mediated CD73 Knockout in Pancreatic Cancer

Zhang J, Zhang S, Dörflein I, Ren X, Pfeffer S, Britzen-Laurent N, Grützmann R, Duan X, Pilarsky C (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Cancers MDPI

Book Volume: 15

Article Number: 4842

Journal Issue: 19

DOI: 10.3390/cancers15194842

Abstract

Pancreatic cancer is among the cancers with the highest mortality rates. Most of the patients are found to have advanced cancer, losing the chance of surgical treatment, and there is an urgent need to find new treatment methods. Targeted therapy for specific genes that play a key role in cancer is now an important means to improve the survival rate of patients. We determined that CD73 is highly expressed in pancreatic cancer by flow cytometry and qRT-PCR assays combined with bioinformatics techniques. Application of CRISPR/Cas9 technology to knockout CD73 in human and murine cell lines, respectively, revealed that CD73 inactivation inhibited cell growth and migration and induced G1 cell cycle arrest. We also found that CD73 deletion inhibited the ERK/STAT3 pathway and activated the E-cadherin pathway. In addition, a CRISPR/Cas9 protein kinase library screen was performed and identified Pbk, Fastk, Cdk19, Adck5, Trim28, and Pfkp as possible genes regulating CD73.

Authors with CRIS profile

Jinping Zhang Professur für Chirurgische Forschung Shuman Zhang Professur für Chirurgische Forschung Xiaofan Ren Professur für Chirurgische Forschung Sina Pfeffer Medizinische Fakultät Nathalie Britzen-Laurent Medizinische Fakultät Robert Grützmann Department of Surgery Christian Pilarsky Professur für Chirurgische Forschung

Involved external institutions

Shaanxi Provincial People's Hospital / Third Affiliated Hospital of Xi'an Jiaotong University / 陕西省人民医院 CN China (CN)

How to cite

APA:

Zhang, J., Zhang, S., Dörflein, I., Ren, X., Pfeffer, S., Britzen-Laurent, N.,... Pilarsky, C. (2023). Impact of CRISPR/Cas9-Mediated CD73 Knockout in Pancreatic Cancer. Cancers, 15(19). https://doi.org/10.3390/cancers15194842

MLA:

Zhang, Jinping, et al. "Impact of CRISPR/Cas9-Mediated CD73 Knockout in Pancreatic Cancer." Cancers 15.19 (2023).

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