Durability assessment of hydrogel mountings for contrast-enhanced micro-CT
Hildebrand T, Novak J, Nogueira LP, Boccaccini AR, Haugen HJ (2023)
Publication Type: Journal article
Publication year: 2023
Journal
Book Volume: 174
Article Number: 103533
DOI: 10.1016/j.micron.2023.103533
Abstract
Micro-computed tomography (micro-CT) provides valuable data for studying soft tissue, though it is often affected by sample movement during scans and low contrast in X-ray absorption. This can result in lower image quality and geometric inaccuracies, collectively known as 'artefacts'. To mitigate these issues, samples can be embedded in hydrogels and enriched with heavy metals for contrast enhancement. However, the long-term durability of these enhancements remains largely unexplored. In this study, we examine the effects of two contrast enhancement agents – iodine and phosphotungstic acid (PTA) – and two hydrogels – agarose and Poloxamer 407 – over a 14-day period. We used Drosophila melanogaster as a test model for our investigation. Our findings reveal that PTA and agarose are highly durable, while iodine and poloxamer hydrogel exhibits higher leakage rates. These observations lay the foundation for estimating contrast stabilities in contrast-enhanced micro-CT with hydrogel embedding and serve to inform future research in this field.
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Norway (NO)How to cite
APA:
Hildebrand, T., Novak, J., Nogueira, L.P., Boccaccini, A.R., & Haugen, H.J. (2023). Durability assessment of hydrogel mountings for contrast-enhanced micro-CT. Micron, 174. https://dx.doi.org/10.1016/j.micron.2023.103533
MLA:
Hildebrand, Torben, et al. "Durability assessment of hydrogel mountings for contrast-enhanced micro-CT." Micron 174 (2023).
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