Precision fermentation as a route to modify β-lactoglobulin structure through substitution of specific cysteine residues

Brune SE, Hoppenreijs LJ, Kuehl T, Lautenbach V, Walter J, Peukert W, Schwarz K, Imhof D, Boom RM, Krull R, Keppler JK, Biedendieck R (2023)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2023

Journal

International Dairy Journal Elsevier

Publisher: Elsevier Ltd

Book Volume: 147

Article Number: 105772

DOI: 10.1016/j.idairyj.2023.105772

Abstract

Precision fermentation is a promising route to develop recombinant proteins with improved functionality. Protein functionality is highly influenced by protein structure, which can be significantly affected by conformational restraints induced by cysteines. Here, we study the impact of individual cysteines on β-lactoglobulin structure, by stepwise substituting them with alanine. Structural characterisation was performed from secondary to quaternary level. The findings demonstrated that the free Cys121 played no role in the folding and dimerisation of BLG. The β-barrel usually includes one disulphide bond (Cys106–Cys119), which was found to be crucial for its formation. The usually exposed disulphide bond (Cys66–Cys160) played no major role in folding of the β-barrel, but mainly modulated its accessibility and mediated dimerisation. Thereby, cysteine mutations can be used as a tool to modify protein structures. These different structures can now be used to further understand the impact on protein behaviour and their functionality.

Authors with CRIS profile

Vanessa Lautenbach Lehrstuhl für Partikelsynthese (LFG) Johannes Walter Functional Particle Systems (FPS) Wolfgang Peukert Lehrstuhl für Partikelsynthese (LFG)

Involved external institutions

Wageningen University & Research NL Netherlands (NL) Christian-Albrechts-Universität zu Kiel DE Germany (DE) Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE) Technische Universität Braunschweig DE Germany (DE)

How to cite

APA:

Brune, S.E., Hoppenreijs, L.J., Kuehl, T., Lautenbach, V., Walter, J., Peukert, W.,... Biedendieck, R. (2023). Precision fermentation as a route to modify β-lactoglobulin structure through substitution of specific cysteine residues. International Dairy Journal, 147. https://doi.org/10.1016/j.idairyj.2023.105772

MLA:

Brune, Sarah E., et al. "Precision fermentation as a route to modify β-lactoglobulin structure through substitution of specific cysteine residues." International Dairy Journal 147 (2023).

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