5-aminolevulinic acid-induced protoporphyrin IX levels in tissue of human malignant brain tumors

Johansson A, Palte G, Schnell O, Tonn JC, Herms J, Stepp H (2010)

Publication Type: Journal article

Publication year: 2010

Journal

Photochemistry and Photobiology Wiley-Blackwell

Book Volume: 86

Pages Range: 1373-1378

Journal Issue: 6

DOI: 10.1111/j.1751-1097.2010.00799.x

Abstract

Protoporphyrin IX (PpIX) produced from exogenous, orally administered 5-aminolevulinic acid (ALA) displays high tumor-selective uptake and is being successfully employed for fluorescence-guided resection (FGR) of human malignant gliomas. Furthermore, the phototoxicity of PpIX can be utilized for photodynamic therapy (PDT) of brain tumors, which has been shown previously. Here, the absolute PpIX concentration in human brain tissue was investigated following oral ALA administration (20 mg kg-1 b.w.). An extraction procedure was used to quantify PpIX in macroscopic tissue samples, weighing 0.013-0.214 g, obtained during FGR. The PpIX concentration was significantly higher in vital grade IV tumors (5.8 ± 4.8 μm, mean ± SD, range 0-28.2 μm, n = 8) as compared with grade III tumors (0.2 ± 0.4 μm, mean ± SD, range 0-0.9 μm, n = 4). There was also a large heterogeneity within grade IV tumors with PpIX displaying significantly lower levels in infiltration zones and necrotic regions as compared with vital tumor parts. The average PpIX concentration in vital grade IV tumor parts was in the range previously shown sufficient for PDT-induced tissue damage following irradiation. However, the feasibility of PDT for grade III brain tumors and for grade IV brain tumors displaying mainly necrotic tissue areas without solid tumor parts needs to be further investigated. © 2010 The Authors. Journal Compilation. The American Society of Photobiology.

Authors with CRIS profile

Oliver Schnell

Involved external institutions

Klinikum der Universität München DE Germany (DE)

How to cite

APA:

Johansson, A., Palte, G., Schnell, O., Tonn, J.C., Herms, J., & Stepp, H. (2010). 5-aminolevulinic acid-induced protoporphyrin IX levels in tissue of human malignant brain tumors. Photochemistry and Photobiology, 86(6), 1373-1378. https://dx.doi.org/10.1111/j.1751-1097.2010.00799.x

MLA:

Johansson, Ann, et al. "5-aminolevulinic acid-induced protoporphyrin IX levels in tissue of human malignant brain tumors." Photochemistry and Photobiology 86.6 (2010): 1373-1378.

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