Adhesion GPCR Gpr126 (Adgrg6) Expression Profiling in Zebrafish, Mouse, and Human Kidney.

Cazorla-Vázquez S, Kösters P, Bertz S, Pfister F, Daniel C, Dedden M, Zundler S, Jobst-Schwan T, Amann KU, Engel F (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Cells MDPI

Book Volume: 12

Pages Range: 1988

Journal Issue: 15

DOI: 10.3390/cells12151988

Abstract

Adhesion G protein-coupled receptors (aGPCRs) comprise the second-largest class of GPCRs, the most common target for approved pharmacological therapies. aGPCRs play an important role in development and disease and have recently been associated with the kidney. Several aGPCRs are expressed in the kidney and some aGPCRs are either required for kidney development or their expression level is altered in diseased kidneys. Yet, general aGPCR function and their physiological role in the kidney are poorly understood. Here, we characterize in detail Gpr126 (Adgrg6) expression based on RNAscope® technology in zebrafish, mice, and humans during kidney development in adults. Gpr126 expression is enriched in the epithelial linage during nephrogenesis and persists in the adult kidney in parietal epithelial cells, collecting ducts, and urothelium. Single-cell RNAseq analysis shows that gpr126 expression is detected in zebrafish in a distinct ionocyte sub-population. It is co-detected selectively with slc9a3.2, slc4a4a, and trpv6, known to be involved in apical acid secretion, buffering blood or intracellular pH, and to maintain high cytoplasmic Ca2+ concentration, respectively. Furthermore, gpr126-expressing cells were enriched in the expression of potassium transporter kcnj1a.1 and gcm2, which regulate the expression of a calcium sensor receptor. Notably, the expression patterns of Trpv6, Kcnj1a.1, and Gpr126 in mouse kidneys are highly similar. Collectively, our approach permits a detailed insight into the spatio-temporal expression of Gpr126 and provides a basis to elucidate a possible role of Gpr126 in kidney physiology.

Authors with CRIS profile

Peter Kösters Professur für Experimentelle Nieren- und Kreislaufforschung Simone Bertz Institute of Pathology Frederick Pfister Medizinische Fakultät Christoph Daniel Department of Nephropathology Mark Dedden Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Sebastian Zundler Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Tilman Jobst-Schwan Department of Medicine 4 – Nephrology and Hypertension Kerstin Ute Amann Department of Nephropathology Felix Engel Professur für Experimentelle Nieren- und Kreislaufforschung

Additional Organisation(s)

Interdisziplinäres Zentrum für Klinische Forschung

Related research project(s)

F007: Gpr126 (Adgrg6) in kidney development and disease May 1, 2020 - April 30, 2023

How to cite

APA:

Cazorla-Vázquez, S., Kösters, P., Bertz, S., Pfister, F., Daniel, C., Dedden, M.,... Engel, F. (2023). Adhesion GPCR Gpr126 (Adgrg6) Expression Profiling in Zebrafish, Mouse, and Human Kidney. Cells, 12(15), 1988. https://doi.org/10.3390/cells12151988

MLA:

Cazorla-Vázquez, Salvador, et al. "Adhesion GPCR Gpr126 (Adgrg6) Expression Profiling in Zebrafish, Mouse, and Human Kidney." Cells 12.15 (2023): 1988.

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