Sharafutdinov I, Ekici AB, Vieth M, Backert S, Linz B (2022)
Publication Type: Journal article
Publication year: 2022
Book Volume: 1
Pages Range: 100032
Article Number: 100032
Issue: 3
DOI: 10.1016/j.cellin.2022.100032
Infection of the stomach by Helicobacter pylori is a major risk factor for the development of gastric cancer. Colonization of the gastric epithelium leads to the activation of multiple disease-related signaling pathways. Serine protease HtrA represents an important secreted virulence factor that mediates cleavage of cellular junctions. However, its potential role in nuclear responses is unknown. Here, we performed a genome-wide RNA-seq analysis of polarized gastric epithelial cells infected by wild-type (wt) and ΔhtrA mutant bacteria. Fluorescence microscopy showed that H. pylori wt, but not ΔhtrA bacteria, preferably localized at cellular junctions. Our results pinpointed early (2 h) and late (6 h) transcriptional responses, with most differentially expressed genes at 6 h post infection. The transcriptomes revealed HtrA-dependent targeting of genes associated with inflammation and apoptosis (e.g. IL8, ZFP36, TNF). Accordingly, infection with the ΔhtrA mutant induced increased apoptosis rates in host cells, which was associated with reduced H. pylori CagA expression. In contrast, transcription of various carcinogenesis-associated genes (e.g. DKK1, DOCK8) was affected by H. pylori independent of HtrA. These findings suggest that H. pylori disturbs previously unknown molecular pathways in an HtrA-dependent and HtrA-independent manner, and provide valuable new insights of this significant pathogen in humans and thus potential targets for better controlling the risk of malignant transformation.
APA:
Sharafutdinov, I., Ekici, A.B., Vieth, M., Backert, S., & Linz, B. (2022). Early and late genome-wide gastric epithelial transcriptome response during infection with the human carcinogen Helicobacter pylori. Cell Insight, 1, 100032. https://doi.org/10.1016/j.cellin.2022.100032
MLA:
Sharafutdinov, Irshad, et al. "Early and late genome-wide gastric epithelial transcriptome response during infection with the human carcinogen Helicobacter pylori." Cell Insight 1 (2022): 100032.
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