Magnetic Removal of Candida albicans Using Salivary Peptide-Functionalized SPIONs

Friedrich B, Tietze R, Dümig M, Sover A, Boca MA, Schreiber E, Band J, Janko C, Krappmann S, Alexiou C, Lyer S (2023)

Publication Type: Journal article

Publication year: 2023

Journal

International Journal of Nanomedicine Dove Medical Press

Book Volume: 18

Pages Range: 3231-3246

DOI: 10.2147/IJN.S409559

Abstract

Purpose: Magnetic separation of microbes can be an effective tool for pathogen identification and diagnostic applications to reduce the time needed for sample preparation. After peptide functionalization of superparamagnetic iron oxide nanoparticles (SPIONs) with an appropriate interface, they can be used for the separation of sepsis-associated yeasts like Candida albicans. Due to their magnetic properties, the magnetic extraction of the particles in the presence of an external magnetic field ensures the accumulation of the targeted yeast. Materials and Methods: In this study, we used SPIONs coated with 3-aminopropyltriethoxysilane (APTES) and functionalized with a peptide originating from GP340 (SPION-APTES-Pep). For the first time, we investigate whether this system is suitable for the separation and enrichment of Candida albicans, we investigated its physicochemical properties and by thermogravimetric analysis we determined the amount of peptide on the SPIONs. Further, the toxicological profile was evaluated by recording cell cycle and DNA degradation. The separation efficiency was investigated using Candida albicans in different experimental settings, and regrowth experiments were carried out to show the use of SPION-APTES-Pep as a sample preparation method for the identification of fungal infections. Conclusion: SPION-APTES-Pep can magnetically remove more than 80% of the microorganism and with a high selective host-pathogen distinction Candida albicans from water-based media and about 55% in blood after 8 minutes processing without compromising effects on the cell cycle of human blood cells. Moreover, the separated fungal cells could be regrown without any restrictions.

Authors with CRIS profile

Bernhard Friedrich Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christina Janko Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Sven Krappmann Professur für Klinische Mikrobiologie und Immunologie Christoph Alexiou Medizinische Fakultät Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik)

Involved external institutions

Hochschule Ansbach DE Germany (DE)

How to cite

APA:

Friedrich, B., Tietze, R., Dümig, M., Sover, A., Boca, M.A., Schreiber, E.,... Lyer, S. (2023). Magnetic Removal of Candida albicans Using Salivary Peptide-Functionalized SPIONs. International Journal of Nanomedicine, 18, 3231-3246. https://doi.org/10.2147/IJN.S409559

MLA:

Friedrich, Bernhard, et al. "Magnetic Removal of Candida albicans Using Salivary Peptide-Functionalized SPIONs." International Journal of Nanomedicine 18 (2023): 3231-3246.

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