Purification and concentration of infectious koi herpesvirus using steric exclusion chromatography

Eilts F, Jordan L, Harsy YM, Bergmann SM, Becker A, Wolff MW (2023)

Publication Type: Journal article, Original article

Publication year: 2023

Journal

Journal of Fish Diseases Wiley-Blackwell

Pages Range: 1-14

DOI: 10.1111/jfd.13800

Open Access Link: https://doi.org/10.1111/jfd.13800

Abstract

Koi herpesvirus (KHV) is the causative agent of a koi herpesvirus disease (KHVD)
inducing high mortality rates in common carp and koi (Cyprinus carpio). No widespread
effective vaccination strategy has been implemented yet, which is partly due to side
effects of the immunized fish. In this study, we present an evaluation of the purification
of infectious KHV from host cell protein and DNA, using the steric exclusion
chromatography. The method is related to conventional polyethylene glycol (PEG)
precipitation implemented in a chromatographic set-up
and has been applied for infectious
virus particle purification with high recoveries and impurity removal. Here, we
achieved a yield of up to 55% of infectious KHV by using 12% PEG (molecular weight
of 6 kDa) at pH 7.0. The recoveries were higher when using chromatographic cellulose
membranes with 3–5 μm pores in diameter instead of 1 μm. The losses were assumed
to originate from dense KHV precipitates retained on the membranes. Additionally,
the use of >0.6 M NaCl was shown to inactivate infectious KHV. In summary, we propose
a first step towards a purification procedure for infectious KHV with a possible
implementation in fish vaccine manufacturing.

Authors with CRIS profile

Lisa Jordan Technische Fakultät Anna Becker Lehrstuhl für Bioverfahrenstechnik

Involved external institutions

Technische Hochschule Mittelhessen - University of Applied Sciences DE Germany (DE) Friedrich-Loeffler-Institut (FLI), Bundesforschungsinstitut für Tiergesundheit DE Germany (DE)

How to cite

APA:

Eilts, F., Jordan, L., Harsy, Y.M., Bergmann, S.M., Becker, A., & Wolff, M.W. (2023). Purification and concentration of infectious koi herpesvirus using steric exclusion chromatography. Journal of Fish Diseases, 1-14. https://dx.doi.org/10.1111/jfd.13800

MLA:

Eilts, Friederike, et al. "Purification and concentration of infectious koi herpesvirus using steric exclusion chromatography." Journal of Fish Diseases (2023): 1-14.

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