Initial surface roughness influence on the generation of LIPSS on titanium and stainless steel and their effect on cell/bacteria viability
Sotelo L, Fontanot T, Vig S, Fernandes MH, Sarau G, Leuchs G, Christiansen S (2023)
Publication Type: Conference contribution
Publication year: 2023
Publisher: SPIE
Book Volume: 12409
Conference Proceedings Title: Proceedings of SPIE - The International Society for Optical Engineering
Event location: San Francisco, CA, USA
ISBN: 9781510659230
DOI: 10.1117/12.2648921
Abstract
In this work, the influence of initial surface roughness on laser-induced periodic surface structures (LIPSS) formation is explored for titanium and stainless steel samples polished with grain sizes of 18.3µm, 8.4µm, 5µm, and 0.5µm, and lasered maintaining the same irradiation conditions. The resulting structures were studied by scanning electron microscopy (SEM), atomic force microscopy (AFM), Raman spectroscopy, and contact angle (CA) measurements, in order to characterize LIPSS periodicity and orientation, as well as surface chemistry and wettability. After characterization, representative structures were chosen to further explore their potential for bone implant treatment by inducing cells (MG63) and bacteria (E.coli and S.aureus) and testing for viability by resazurin assays, alkaline phosphatase activity, and SEM imaging. Results show that initial surface roughness (Ra) plays a different role on LIPSS generation for both materials, with stainless steel showing a higher dependence on Ra than titanium, however, both materials show a reduction on bacterial viability, while cell proliferation between polished and lasered samples also show an enhanced osteogenic effect.
Authors with CRIS profile
Involved external institutions
Innovations-Institut für Nanotechnologie und korrelative Mikroskopie e.V. (INAM) / Institute for Nanotechnology and Correlative Microscopy
Germany (DE)
Fraunhofer-Institut für Keramische Technologien und Systeme (IKTS)
Germany (DE)
Universidade do Porto
Portugal (PT)
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
Germany (DE)
Fraunhofer-Institut für Keramische Technologien und Systeme (IKTS)
Germany (DE)
Universidade do Porto
Portugal (PT)
Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light
Germany (DE)
How to cite
APA:
Sotelo, L., Fontanot, T., Vig, S., Fernandes, M.H., Sarau, G., Leuchs, G., & Christiansen, S. (2023). Initial surface roughness influence on the generation of LIPSS on titanium and stainless steel and their effect on cell/bacteria viability. In Rainer Kling, Akira Watanabe, Wilhelm Pfleging (Eds.), Proceedings of SPIE - The International Society for Optical Engineering. San Francisco, CA, USA: SPIE.
MLA:
Sotelo, Lamborghini, et al. "Initial surface roughness influence on the generation of LIPSS on titanium and stainless steel and their effect on cell/bacteria viability." Proceedings of the Laser-based Micro- and Nanoprocessing XVII 2023, San Francisco, CA, USA Ed. Rainer Kling, Akira Watanabe, Wilhelm Pfleging, SPIE, 2023.
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