A multiomic approach to defining the essential genome of the globally important pathogen Corynebacterium diphtheriae
Goodall ECAF, Antunes CAA, Möller J, Sangal VR, Torres VVL, Gray J, Cunningham AF, Hoskisson PA, Burkovski A, Henderson IR (2023)
Publication Type: Journal article
Publication year: 2023
Journal
Book Volume: 19
Journal Issue: 4
DOI: 10.1371/journal.pgen.1010737
Abstract
Author summaryCorynebacterium diphtheriae causes both toxin-mediated diphtheria and non-toxigenic invasive infections. Despite a vaccine to protect against diphtheria, case numbers for both invasive and diphtherial disease have increased over the last decade. Furthermore, an increase in antibiotic resistant strains are being isolated from patients. It's clear that additional treatment strategies for this organism will be needed in the future. Using high-throughput mutagenesis, this work presents the densest library of mutants for any Corynebacterium sp.. This work identifies the essential genome of C. diphtheriae; an important classification as these genes are often the target of therapeutic intervention. We identify highly conserved genes and species-specific genes unique to pathogens. This data presents an important benchmark and focus for the future development of therapeutic options. Of particular significance is the identification of uncharacterized, conserved proteins within the Diphtheria vaccine.
Authors with CRIS profile
Involved external institutions
Deutsches Krebsforschungszentrum (DKFZ)
Germany (DE)
University of Queensland
Australia (AU)
Northumbria University
United Kingdom (GB)
University of Strathclyde
United Kingdom (GB)
University of Birmingham
United Kingdom (GB)
Germany (DE)
University of Queensland
Australia (AU)
Northumbria University
United Kingdom (GB)
University of Strathclyde
United Kingdom (GB)
University of Birmingham
United Kingdom (GB)
How to cite
APA:
Goodall, E.C.A.F., Antunes, C.A.A., Möller, J., Sangal, V.R., Torres, V.V.L., Gray, J.,... Henderson, I.R. (2023). A multiomic approach to defining the essential genome of the globally important pathogen Corynebacterium diphtheriae. PLoS Genetics, 19(4). https://dx.doi.org/10.1371/journal.pgen.1010737
MLA:
Goodall, Emily C. A. F., et al. "A multiomic approach to defining the essential genome of the globally important pathogen Corynebacterium diphtheriae." PLoS Genetics 19.4 (2023).
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