FUCCI Reporter Gene-Based Cell Cycle Analysis

Fischer L, Thievessen I (2023)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2023


Publisher: Humana Press Inc.

Series: Methods in Molecular Biology

Book Volume: 2644

Pages Range: 371-385

DOI: 10.1007/978-1-0716-3052-5_24


One of the most commonly assessed parameters in cellular analyses is the proliferative activity of a cell population. The fluorescence ubiquitin cell cycle indicator (FUCCI)-based system allows live and in vivo observation of cell cycle progression. Based on the mutually exclusive activity of two fluorescently labeled proteins cdt1 and geminin during the G0/1 and S/G2/M phases of the cell cycle, individual cells can be assigned to their respective cell cycle phase by fluorescence imaging of the nucleus. Here, we describe the generation of NIH/3T3 cells containing the FUCCI reporter system by lentiviral transduction and their use in 3D culture assays. The protocol can be adapted to other cell lines.

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How to cite


Fischer, L., & Thievessen, I. (2023). FUCCI Reporter Gene-Based Cell Cycle Analysis. In (pp. 371-385). Humana Press Inc..


Fischer, Lena, and Ingo Thievessen. "FUCCI Reporter Gene-Based Cell Cycle Analysis." Humana Press Inc., 2023. 371-385.

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