Cellular density and variability in laryngeal and pharyngeal squamous cell carcinoma using confocal laser endomicroscopy

Sievert M, Aubreville M, Eckstein M, Mantsopoulos K, Koch M, Gostian AO, Mueller SK, Iro H, Goncalves M (2023)

Publication Type: Journal article

Publication year: 2023

Journal

European Review for Medical and Pharmacological Sciences Verduci Publishers

Book Volume: 27

Pages Range: 3622-3630

Journal Issue: 8

DOI: 10.26355/eurrev_202304_32146

Abstract

OBJECTIVE: Confocal laser endomicroscopy (CLE) allows the visualization of epithelium in a thousand-fold magnification. This study analyzes the architectural differences at the cellular level of the mucosa and squamous cell carcinoma (SCC). PATIENTS AND METHODS: A total of 60 CLE sequences recorded in 5 patients with SCC undergoing laryngectomy between October 2020 and February 2021 were analyzed. The corresponding histologic sample derived from H&E staining was assigned to each sequence, capturing CLE images of the tumor and healthy mucosa. In addition, the cellular structure analysis was performed to diagnose SCC by measuring the total number of cells and cell size in 60 sequences in a fixed field of view (FOV) with 240 μm in diameter (45,239 μm2). RESULTS: Out of 3,600 images, 1,620 (45%) showed benign mucosa and 1,980 (55%) SCC. The automated analysis yielded a difference in cell size, with healthy epithelial cells being 171.9±82.0 μm2 smaller than SCC cells, which were 246.3±171.9 μm2 and showed greater variability in size (p=0.037). In addition, due to the probe’s fixed FOV, there was a difference in cell count with a total of 188.7±38.3 and 124.8±38.6 cells in images of normal epithelium and SCC (p<0.001), respectively. Regarding cell density as a criterion for the differentiation of benign/malign, using a cut-off value of 145.5 cells/FOV, we obtained sensitivity and specificity of 88.0% and 71.9%, respectively. CONCLUSIONS: SCC reveals marked differences at a cellular level compared to the healthy epithelium. Our results further support the importance of this feature for identifying SCC during CLE imaging.

Authors with CRIS profile

Matti Sievert Department of Otorhinolaryngology – Head and Neck Surgery Markus Eckstein Institute of Pathology Konstantinos Mantsopoulos Department of Otorhinolaryngology – Head and Neck Surgery Antoniu-Oreste Gostian Department of Otorhinolaryngology – Head and Neck Surgery Sarina Müller-Hübner Department of Otorhinolaryngology – Head and Neck Surgery Heinrich Iro Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde

Involved external institutions

Technische Hochschule Ingolstadt DE Germany (DE) Universitätsklinikum Aachen (UKA) DE Germany (DE)

How to cite

APA:

Sievert, M., Aubreville, M., Eckstein, M., Mantsopoulos, K., Koch, M., Gostian, A.-O.,... Goncalves, M. (2023). Cellular density and variability in laryngeal and pharyngeal squamous cell carcinoma using confocal laser endomicroscopy. European Review for Medical and Pharmacological Sciences, 27(8), 3622-3630. https://doi.org/10.26355/eurrev_202304_32146

MLA:

Sievert, Matti, et al. "Cellular density and variability in laryngeal and pharyngeal squamous cell carcinoma using confocal laser endomicroscopy." European Review for Medical and Pharmacological Sciences 27.8 (2023): 3622-3630.

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