Immobilization of Aspergillus sp. laccase on hierarchical silica MFI zeolite with embedded macropores

Tocco D, Wisser D, Fischer M, Schwieger W, Salis A, Hartmann M (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Colloids and Surfaces B: Biointerfaces Elsevier

Book Volume: 226

Article Number: 113311

DOI: 10.1016/j.colsurfb.2023.113311

Abstract

Laccase from Aspergillus sp. (LC) was immobilized on functionalized silica hierarchical (microporous-macroporous) MFI zeolite (ZMFI). The obtained immobilized biocatalyst (LC#ZMFI) was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (ATR-FTIR), N2 adsorption/desorption isotherms, solid-state NMR spectroscopy and thermogravimetric analysis (TGA) confirming the chemical anchoring of the enzyme to the zeolitic support. The optimal pH, kinetic parameters (KM and Vmax), specific activity, as well as both storage and operational stability of LC#ZMFI were determined. The LC#ZMFI KM and Vmax values amount to 10.3 µM and 0.74 µmol·mg−1 min−1, respectively. The dependence of specific activity on the pH for free and immobilized LC was investigated in the pH range of 2–7, The highest specific activity was obtained at pH = 3 for both free LC and LC#ZMFI. LC#ZMFI retained up to 50 % and 30 % of its original activity after storage of 21 and 30 days, respectively. Immobilization of laccase on hierarchical silica MFI zeolite allows to carry out the reaction under acidic pH values without affecting the support structure.

Authors with CRIS profile

Davide Tocco Professur für Katalyse Dorothea Wisser Interdisziplinäres Zentrum Erlangen Catalysis Resource Center (ECRC) Marcus Fischer Interdisziplinäres Zentrum Erlangen Catalysis Resource Center (ECRC) Wilhelm Schwieger Professur für Technische Chemie (Reaktionstechnik) Martin Hartmann Professur für Katalyse

Involved external institutions

Università degli Studi di Cagliari IT Italy (IT)

How to cite

APA:

Tocco, D., Wisser, D., Fischer, M., Schwieger, W., Salis, A., & Hartmann, M. (2023). Immobilization of Aspergillus sp. laccase on hierarchical silica MFI zeolite with embedded macropores. Colloids and Surfaces B: Biointerfaces, 226. https://doi.org/10.1016/j.colsurfb.2023.113311

MLA:

Tocco, Davide, et al. "Immobilization of Aspergillus sp. laccase on hierarchical silica MFI zeolite with embedded macropores." Colloids and Surfaces B: Biointerfaces 226 (2023).

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