Raman and Infrared Spectroscopy Distinguishing Replicative Senescent from Proliferating Primary Human Fibroblast Cells by Detecting Spectral Differences Mainly Due to Biomolecular Alterations
Eberhardt K, Beleites C, Marthandan S, Matthaeus C, Diekmann S, Popp J (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 89
Pages Range: 2937-2947
Journal Issue: 5
DOI: 10.1021/acs.analchem.6b04264
Abstract
Cellular senescence is a terminal cell cycle arrested state, assumed to be involved in tumor suppression. We studied four human fibroblast cell strains (BJ, MRC-5, IMR-90, and WI-38) from proliferation into senescence. Cells were investigated by label-free vibrational Raman and infrared spectroscopy, following their transition into replicative senescence. During the transition into senescence, we observed rather similar biomolecular abundances in all four cell strains and between proliferating and senescent cells; however, in the four aging cell strains, we found common molecular differences dominated by protein and lipid modifications. Hence, aging induces a change in the appearance of biomolecules (including degradation and storage of waste) rather than in their amount present in the cells. For all fibroblast strains combined, the partial least squares-linear discriminant analysis (PLS-LDA) model resulted in 75% and 81% accuracy for the Raman and infrared (IR) data, respectively. Within this validation, senescent cells were recognized with 93% sensitivity and 90% specificity for the Raman and 84% sensitivity and 97% specificity for the IR data. Thus, Raman and infrared spectroscopy can identify replicative senescence on the single cell level, suggesting that vibrational spectroscopy may be suitable for identifying and distinguishing different cellular states in vivo, e.g., in skin.
Involved external institutions
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut (FLI)
Germany (DE)
Germany (DE)
Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut (FLI)
Germany (DE)
How to cite
APA:
Eberhardt, K., Beleites, C., Marthandan, S., Matthaeus, C., Diekmann, S., & Popp, J. (2017). Raman and Infrared Spectroscopy Distinguishing Replicative Senescent from Proliferating Primary Human Fibroblast Cells by Detecting Spectral Differences Mainly Due to Biomolecular Alterations. Analytical Chemistry, 89(5), 2937-2947. https://dx.doi.org/10.1021/acs.analchem.6b04264
MLA:
Eberhardt, Katharina, et al. "Raman and Infrared Spectroscopy Distinguishing Replicative Senescent from Proliferating Primary Human Fibroblast Cells by Detecting Spectral Differences Mainly Due to Biomolecular Alterations." Analytical Chemistry 89.5 (2017): 2937-2947.
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