Pioneering particle-based strategy for isolating viable bacteria from multipart soil samples compatible with Raman spectroscopy

Schwarz M, Kloss S, Stoeckel S, Pollok S, Hollaender A, Cialla-May D, Weber K, Popp J (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Analytical and Bioanalytical Chemistry Springer

Book Volume: 409

Pages Range: 3779-3788

Journal Issue: 15

DOI: 10.1007/s00216-017-0320-z

Abstract

The study of edaphic bacteria is of great interest, particularly for evaluating soil remediation and recultivation methods. Therefore, a fast and simple strategy to isolate various bacteria from complex soil samples using poly(ethyleneimine) (PEI)-modified polyethylene particles is introduced. The research focuses on the binding behavior under different conditions, such as the composition, pH value, and ionic strength, of the binding buffer, and is supported by the characterization of the surface properties of particles and bacteria. The results demonstrate that electrostatic forces and hydrophobicity are responsible for the adhesion of target bacteria to the particles. Distinct advantages of the particle-based isolation strategy include simple handling, enrichment efficiency, and the preservation of viable bacteria. The presented isolation method allows a subsequent identification of the bacteria using Raman microspectroscopy in combination with chemometrical methods. This is demonstrated with a dataset of five different bacteria (Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, Streptomyces tendae, and Streptomyces acidiscabies) which were isolated from spiked soil samples. In total 92% of the Raman spectra could be identified correctly.

Involved external institutions

Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) InfectoGnostics Forschungscampus Jena e. V. DE Germany (DE) Technische Hochschule Mittelhessen - University of Applied Sciences DE Germany (DE) Fraunhofer-Institut für Angewandte Polymerforschung (IAP) / Fraunhofer Institute for Applied Polymer Research DE Germany (DE)

How to cite

APA:

Schwarz, M., Kloss, S., Stoeckel, S., Pollok, S., Hollaender, A., Cialla-May, D.,... Popp, J. (2017). Pioneering particle-based strategy for isolating viable bacteria from multipart soil samples compatible with Raman spectroscopy. Analytical and Bioanalytical Chemistry, 409(15), 3779-3788. https://dx.doi.org/10.1007/s00216-017-0320-z

MLA:

Schwarz, Martha, et al. "Pioneering particle-based strategy for isolating viable bacteria from multipart soil samples compatible with Raman spectroscopy." Analytical and Bioanalytical Chemistry 409.15 (2017): 3779-3788.

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