Uptake of Retinoic Acid-Modified PMMA Nanoparticles in LX-2 and Liver Tissue by Raman Imaging and Intravital Microscopy
Yildirim T, Matthaeus C, Press AT, Schubert S, Bauer M, Popp J, Schubert US (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 17
Article Number: 1700064
Journal Issue: 10
Abstract
A primary amino-functionalized methyl methacrylate-based statistical copolymer is covalently coupled with retinoic acid (RA) and a fluorescent dye (DY590) in order to investigate the feasibility of the RA containing polymeric nanoparticles for Raman imaging studies and to study the possible selectivity of RA for hepatic stellate cells via intravital microscopy. Cationic nanoparticles are prepared by utilizing the nanoprecipitation method using modified polymers. Raman studies show that RA functional nanoparticles can be detectable in all tested cells without any need of additional label. Moreover, intravital microscopy indicates that DY590 is eliminated through the hepatobiliary route but not if used as covalently attached tracing molecule for nanoparticles. However, it is a suitable probe for sensitive detection of polymeric nanoparticles.
Involved external institutions
Germany (DE)How to cite
APA:
Yildirim, T., Matthaeus, C., Press, A.T., Schubert, S., Bauer, M., Popp, J., & Schubert, U.S. (2017). Uptake of Retinoic Acid-Modified PMMA Nanoparticles in LX-2 and Liver Tissue by Raman Imaging and Intravital Microscopy. Macromolecular Bioscience, 17(10). https://doi.org/10.1002/mabi.201700064
MLA:
Yildirim, Turgay, et al. "Uptake of Retinoic Acid-Modified PMMA Nanoparticles in LX-2 and Liver Tissue by Raman Imaging and Intravital Microscopy." Macromolecular Bioscience 17.10 (2017).
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