High-Throughput Screening Raman Spectroscopy Platform for Label-Free Cellomics
Schie IW, Rueger J, Mondol AS, Ramoji A, Neugebauer U, Krafft C, Popp J (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 90
Pages Range: 2023-2030
Journal Issue: 3
DOI: 10.1021/acs.analchem.7b04127
Abstract
We present a high-throughput screening Raman spectroscopy (HTS-RS) platform for a rapid and label-free macromolecular fingerprinting of tens of thousands eukaryotic cells. The newly proposed label-free HTS-RS platform combines automated imaging microscopy with Raman spectroscopy to enable a rapid label-free screening of cells and can be applied to a large number of biomedical and clinical applications. The potential of the new approach is illustrated by two applications. (1) HTS-RS-based differential white blood cell count. A classification model was trained using Raman spectra of 52※218 lymphocytes, 48※220 neutrophils, and 7※294 monocytes from four volunteers. The model was applied to determine a WBC differential for two volunteers and three patients, producing comparable results between HTS-RS and machine counting. (2) HTS-RS-based identification of circulating tumor cells (CTCs) in 1:1, 1:9, and 1:99 mixtures of Panc1 cells and leukocytes yielded ratios of 55:45, 10:90, and 3:97, respectively. Because the newly developed HTS-RS platform can be transferred to many existing Raman devices in all laboratories, the proposed implementation will lead to a significant expansion of Raman spectroscopy as a standard tool in biomedical cell research and clinical diagnostics.
Involved external institutions
Germany (DE)How to cite
APA:
Schie, I.W., Rueger, J., Mondol, A.S., Ramoji, A., Neugebauer, U., Krafft, C., & Popp, J. (2018). High-Throughput Screening Raman Spectroscopy Platform for Label-Free Cellomics. Analytical Chemistry, 90(3), 2023-2030. https://doi.org/10.1021/acs.analchem.7b04127
MLA:
Schie, Iwan W., et al. "High-Throughput Screening Raman Spectroscopy Platform for Label-Free Cellomics." Analytical Chemistry 90.3 (2018): 2023-2030.
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