In Vitro Selection of Specific DNA Aptamers Against the Anti-Coagulant Dabigatran Etexilate

Aljohani MM, Chinnappan R, Eissa S, Alsager OA, Weber K, Cialla-May D, Popp J, Zourob M (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 8

Article Number: 13290

Journal Issue: 1

DOI: 10.1038/s41598-018-31327-3

Abstract

Dabigatran Etexilate (PRADAXA) is a new oral anticoagulant increasingly used for a number of blood thrombosis conditions, prevention of strokes and systemic emboli among patients with atrial fibrillation. It provides safe and adequate anticoagulation for prevention and treatment of thrombus in several clinical settings. However, anticoagulation therapy can be associated with an increased risk of bleeding. There is a lack of specific laboratory tests to determine the level of this drug in blood. This is considered the most important obstacles of using this medication, particularly for patients with trauma, drug toxicity, in urgent need for surgical interventions or uncontrolled bleeding. In this work, we performed Systematic evolution of ligands by exponential enrichment (SELEX) to select specific DNA aptamers against dabigatran etexilate. Following multiple rounds of selection and enrichment with a randomized 60-mer DNA library, specific DNA aptamers for dabigatran were selected. We investigated the affinity and specificity of generated aptamers to the drug showing dissociation constants (Kd) ranging from 46.8–208 nM. The most sensitive aptamer sequence was selected and applied in an electrochemical biosensor to successfully achieve 0. 01 ng/ml level of detection of the target drug. With further improvement of the assay and optimization, these aptamers would replace conventional antibodies for developing detection assays in the near future.

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE) Alfaisal University SA Saudi Arabia (SA) King Abdulaziz City for Science and Technology SA Saudi Arabia (SA)

How to cite

APA:

Aljohani, M.M., Chinnappan, R., Eissa, S., Alsager, O.A., Weber, K., Cialla-May, D.,... Zourob, M. (2018). In Vitro Selection of Specific DNA Aptamers Against the Anti-Coagulant Dabigatran Etexilate. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-31327-3

MLA:

Aljohani, Maher M., et al. "In Vitro Selection of Specific DNA Aptamers Against the Anti-Coagulant Dabigatran Etexilate." Scientific Reports 8.1 (2018).

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