FLIm and raman spectroscopy for investigating biochemical changes of bovine pericardium upon genipin cross-linking

Shaik TA, Alfonso-Garcia A, Richter M, Korinth F, Krafft C, Marcu L, Popp J (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Molecules MDPI

Book Volume: 25

Article Number: 25173857

Journal Issue: 17

DOI: 10.3390/molecules25173857

Abstract

Biomaterials used in tissue engineering and regenerative medicine applications benefit from longitudinal monitoring in a non-destructive manner. Label-free imaging based on fluorescence lifetime imaging (FLIm) and Raman spectroscopy were used to monitor the degree of genipin (GE) cross-linking of antigen-removed bovine pericardium (ARBP) at three incubation time points (0.5, 1.0, and 2.5 h). Fluorescence lifetime decreased and the emission spectrum redshifted compared to that of uncross-linked ARBP. The Raman signature of GE-ARBP was resonance-enhanced due to the GE cross-linker that generated new Raman bands at 1165, 1326, 1350, 1380, 1402, 1470, 1506, 1535, 1574, 1630, 1728, and 1741 cm−1. These were validated through density functional theory calculations as cross-linker-specific bands. A multivariate multiple regression model was developed to enhance the biochemical specificity of FLIm parameters fluorescence intensity ratio (R2 = 0.92) and lifetime (R2 = 0.94)) with Raman spectral results. FLIm and Raman spectroscopy detected biochemical changes occurring in the collagenous tissue during the cross-linking process that were characterized by the formation of a blue pigment which affected the tissue fluorescence and scattering properties. In conclusion, FLIm parameters and Raman spectroscopy were used to monitor the degree of cross-linking non-destructively.

Involved external institutions

Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) University of California Davis (UCDAVIS) US United States (USA) (US) Friedrich-Schiller-Universität Jena DE Germany (DE)

How to cite

APA:

Shaik, T.A., Alfonso-Garcia, A., Richter, M., Korinth, F., Krafft, C., Marcu, L., & Popp, J. (2020). FLIm and raman spectroscopy for investigating biochemical changes of bovine pericardium upon genipin cross-linking. Molecules, 25(17). https://doi.org/10.3390/molecules25173857

MLA:

Shaik, Tanveer Ahmed, et al. "FLIm and raman spectroscopy for investigating biochemical changes of bovine pericardium upon genipin cross-linking." Molecules 25.17 (2020).

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