Real-time nanodiamond thermometry probing in vivo thermogenic responses

Fujiwara M, Sun S, Dohms A, Nishimura Y, Suto K, Takezawa Y, Oshimi K, Zhao L, Sadzak N, Umehara Y, Teki Y, Komatsu N, Benson O, Shikano Y, Kage-Nakadai E (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Science Advances American Association for the Advancement of Science

Book Volume: 6

Article Number: eaba9636

Journal Issue: 37

DOI: 10.1126/sciadv.aba9636

Abstract

Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities. However, it is challenging to reduce the size of biocompatible thermometers down to submicrometers, despite their potential applications for the thermal imaging of subtissue structures with single-cell resolution. Here, using quantum nanothermometers based on optically accessible electron spins in nanodiamonds, we demonstrate in vivo real-time temperature monitoring inside Caenorhabditis elegans worms. We developed a microscope system that integrates a quick-docking sample chamber, particle tracking, and an error correction filter for temperature monitoring of mobile nanodiamonds inside live adult worms with a precision of ±0.22°C. With this system, we determined temperature increases based on the worms’ thermogenic responses during the chemical stimuli of mitochondrial uncouplers. Our technique demonstrates the submicrometer localization of temperature information in living animals and direct identification of their pharmacological thermogenesis, which may allow for quantification of their biological activities based on temperature.

Involved external institutions

Osaka City University (OCU) / 大阪市立大学 JP Japan (JP) Humboldt-Universität zu Berlin DE Germany (DE) Soochow University, Suzhou / 苏州大学 CN China (CN) Kyoto University / 京都大学 Kyōto daigaku JP Japan (JP) Keio University / 慶應義塾大学 JP Japan (JP)

How to cite

APA:

Fujiwara, M., Sun, S., Dohms, A., Nishimura, Y., Suto, K., Takezawa, Y.,... Kage-Nakadai, E. (2020). Real-time nanodiamond thermometry probing in vivo thermogenic responses. Science Advances, 6(37). https://doi.org/10.1126/sciadv.aba9636

MLA:

Fujiwara, Masazumi, et al. "Real-time nanodiamond thermometry probing in vivo thermogenic responses." Science Advances 6.37 (2020).

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