Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies
Bocklitz T, Braeutigam K, Urbanek A, Hoffmann F, Von Eggeling F, Ernst G, Schmitt M, Schubert U, Guntinas-Lichius O, Popp J (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 407
Journal Issue: 26
DOI: 10.1007/s00216-015-8987-5
Abstract
Molecular heterogeneity of cancer is a major obstacle in tumor diagnosis and treatment. To deal with this heterogeneity, a multidisciplinary combination of different analysis techniques is of urgent need because a combination enables the creation of a multimodal image of a tumor. Here, we develop a computational workflow in order to combine matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) imaging and Raman microspectroscopic imaging for tissue based studies. The computational workflow can be used to confirm a spectral histopathology (SHP) based on one technique with another technique. In this contribution, we confirmed a Raman spectroscopic based SHP with MALDI-imaging. Owing to this combination, we could demonstrate, for a larynx carcinoma sample, that tissue types and different metabolic states could be extracted from the Raman spectra. Further investigations with the help of MALDI spectra yield a better characterization of variable epithelial differentiation and a better understanding of ongoing dysplastic alterations.
Involved external institutions
Germany (DE)How to cite
APA:
Bocklitz, T., Braeutigam, K., Urbanek, A., Hoffmann, F., Von Eggeling, F., Ernst, G.,... Popp, J. (2015). Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies. Analytical and Bioanalytical Chemistry, 407(26). https://doi.org/10.1007/s00216-015-8987-5
MLA:
Bocklitz, Thomas, et al. "Novel workflow for combining Raman spectroscopy and MALDI-MSI for tissue based studies." Analytical and Bioanalytical Chemistry 407.26 (2015).
BibTeX: Download