Monitoring changes in biochemical and biomechanical properties of collagenous tissues using label-free and nondestructive optical imaging techniques
Shaik TA, Lagarto JL, Baria E, Goktas M, Onoja PI, Blank KG, Pavone FS, Popp J, Krafft C, Cicchi R (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 93
Pages Range: 3813-3821
Journal Issue: 8
DOI: 10.1021/acs.analchem.0c04306
Abstract
We demonstrate the ability of nondestructive optical imaging techniques such as second-harmonic generation (SHG), two-photon fluorescence (TPF), fluorescence lifetime imaging (FLIM), and Raman spectroscopy (RS) to monitor biochemical and mechanical alterations in tissues upon collagen degradation. Decellularized equine pericardium (EP) was treated with 50 μg/mL bacterial collagenase at 37 °C for 8, 16, 24, and 32 h. The SHG ratio (defined as the normalized ratio between SHG and TPF signals) remained unchanged for untreated EP (stored in phosphate-buffered solution (PBS)), whereas treated EP showed a trend of a decreasing SHG ratio with increasing collagen degradation. In the fluorescence domain, treated EP experienced a red-shifted emission and the fluorescence lifetime had a trend of decreasing lifetime with increasing collagen digestion. RS monitors collagen degradation, the spectra had less intense Raman bands at 814, 852, 938, 1242, and 1270 cm−1. Non-negative least-squares (NNLS) modeling quantifies collagen loss and relative increase of elastin. The Young's modulus, derived from atomic force microscope-based nanoindentation experiments, showed a rapid decrease within the first 8 h of collagen degradation, whereas more gradual changes were observed for optical modalities. We conclude that optical imaging techniques like SHG, RS, and FLIM can monitor collagen degradation in a label-free manner and coarsely access mechanical properties in a nondestructive manner.
Involved external institutions
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy
Italy (IT)
Max-Planck-Institut für Kolloid- und Grenzflächenforschung / Max Planck Institute of Colloids and Interfaces
Germany (DE)
Germany (DE)
Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy
Italy (IT)
Max-Planck-Institut für Kolloid- und Grenzflächenforschung / Max Planck Institute of Colloids and Interfaces
Germany (DE)
How to cite
APA:
Shaik, T.A., Lagarto, J.L., Baria, E., Goktas, M., Onoja, P.I., Blank, K.G.,... Cicchi, R. (2021). Monitoring changes in biochemical and biomechanical properties of collagenous tissues using label-free and nondestructive optical imaging techniques. Analytical Chemistry, 93(8), 3813-3821. https://doi.org/10.1021/acs.analchem.0c04306
MLA:
Shaik, Tanveer Ahmed, et al. "Monitoring changes in biochemical and biomechanical properties of collagenous tissues using label-free and nondestructive optical imaging techniques." Analytical Chemistry 93.8 (2021): 3813-3821.
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