In vivo coherent anti-Stokes Raman scattering microscopy reveals vitamin A distribution in the liver

Rodewald M, Bae H, Huschke S, Meyer-Zedler T, Schmitt M, Press AT, Schubert S, Bauer M, Popp J (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Journal of Biophotonics Wiley-VCH Verlag

Book Volume: 14

Article Number: e202100040

Journal Issue: 6

DOI: 10.1002/jbio.202100040

Abstract

Here we present a microscope setup for coherent anti-Stokes Raman scattering (CARS) imaging, devised to specifically address the challenges of in vivo experiments. We exemplify its capabilities by demonstrating how CARS microscopy can be used to identify vitamin A (VA) accumulations in the liver of a living mouse, marking the positions of hepatic stellate cells (HSCs). HSCs are the main source of extracellular matrix protein after hepatic injury and are therefore the main target of novel nanomedical strategies in the development of a treatment for liver fibrosis. Their role in the VA metabolism makes them an ideal target for a CARS-based approach as they store most of the body's VA, a class of compounds sharing a retinyl group as a structural motive, a moiety that is well known for its exceptionally high Raman cross section of the C═C stretching vibration of the conjugated backbone.

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Universitätsklinikum Jena DE Germany (DE)

How to cite

APA:

Rodewald, M., Bae, H., Huschke, S., Meyer-Zedler, T., Schmitt, M., Press, A.T.,... Popp, J. (2021). In vivo coherent anti-Stokes Raman scattering microscopy reveals vitamin A distribution in the liver. Journal of Biophotonics, 14(6). https://doi.org/10.1002/jbio.202100040

MLA:

Rodewald, Marko, et al. "In vivo coherent anti-Stokes Raman scattering microscopy reveals vitamin A distribution in the liver." Journal of Biophotonics 14.6 (2021).

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