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Raman 18O-labeling of bacteria in visible and deep UV-ranges

Matanfack GA, Pistiki A, Roesch P, Popp J (2021)

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Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 14

Article Number: e202100013

Journal Issue: 6

DOI: 10.1002/jbio.202100013

Abstract

Raman stable isotope labeling with ²H, ¹³C or ¹⁵N has been reported as an elegant approach to investigate cellular metabolic activity, which is of great importance to reveal the functions of microorganisms in native environments. A new strategy termed Raman ¹⁸O-labeling was developed to probe the metabolic activity of bacteria. Raman ¹⁸O-labeling refers to the combination of Raman microspectroscopy with ¹⁸O-labeling using H2¹⁸O. At an excitation wavelength of 532 nm, the incorporation of ¹⁸O into the amide I group of proteins and DNA/RNA bases was observed in Escherichia coli cells, while for an excitation wavelength electronically resonant with DNA or aromatic amino acid absorption at 244 nm ¹⁸O assimilation was detected using chemometric tools rather than visual inspection. Raman ¹⁸O-labeling at 532 nm combined with 2D correlation analysis confirmed the assimilation of ¹⁸O in proteins and nucleic acids and revealed the growth strategy of E. coli cells; they underwent protein synthesis followed by nucleic acid synthesis. Independent cultural replicates at different incubation times corroborated the reproducibility of these results. The variations in spectral features of ¹⁸O-labeled cells revealed changes in physiological information of cells. Hence, Raman ¹⁸O-labeling could provide a powerful tool to identify metabolically active bacterial cells.

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How to cite

APA:

Matanfack, G.A., Pistiki, A., Roesch, P., & Popp, J. (2021). Raman 18O-labeling of bacteria in visible and deep UV-ranges. Journal of Biophotonics, 14(6). https://doi.org/10.1002/jbio.202100013

MLA:

Matanfack, Georgette Azemtsop, et al. "Raman 18O-labeling of bacteria in visible and deep UV-ranges." Journal of Biophotonics 14.6 (2021).

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