Isolation of bacteria from artificial bronchoalveolar lavage fluid using density gradient centrifugation and their accessibility by Raman spectroscopy

Wichmann C, Roesch P, Popp J (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Analytical and Bioanalytical Chemistry Springer

Book Volume: 413

Pages Range: 5193-5200

Journal Issue: 20

DOI: 10.1007/s00216-021-03488-0

Abstract

Raman spectroscopy is an analytical method to identify medical samples of bacteria. Because Raman spectroscopy detects the biochemical properties of a cell, there are many factors that can influence and modify the Raman spectra of bacteria. One possible influence is a proper method for isolation of the bacteria. Medical samples in particular never occur in purified form, so a Raman-compatible isolation method is needed which does not affect the bacteria and thus the resulting spectra. In this study, we present a Raman-compatible method for isolation of bacteria from bronchoalveolar lavage (BAL) fluid using density gradient centrifugation. In addition to measuring the bacteria from a patient sample, the yield and the spectral influence of the isolation on the bacteria were investigated. Bacteria isolated from BAL fluid show additional peaks in comparison to pure culture bacteria, which can be attributed to components in the BAL sample. The isolation gradient itself has no effect on the spectra, and with a yield of 63% and 78%, the method is suitable for isolation of low concentrations of bacteria from a complex matrix. [Figure not available: see fulltext.]

Involved external institutions

Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Friedrich-Schiller-Universität Jena DE Germany (DE)

How to cite

APA:

Wichmann, C., Roesch, P., & Popp, J. (2021). Isolation of bacteria from artificial bronchoalveolar lavage fluid using density gradient centrifugation and their accessibility by Raman spectroscopy. Analytical and Bioanalytical Chemistry, 413(20), 5193-5200. https://doi.org/10.1007/s00216-021-03488-0

MLA:

Wichmann, Christina, Petra Roesch, and Juergen Popp. "Isolation of bacteria from artificial bronchoalveolar lavage fluid using density gradient centrifugation and their accessibility by Raman spectroscopy." Analytical and Bioanalytical Chemistry 413.20 (2021): 5193-5200.

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