Multimodal nonlinear endomicroscopic imaging probe using a double-core double-clad fiber and focus-combining micro-optical concept
Pshenay-Severin E, Bae H, Reichwald K, Matz G, Bierlich J, Kobelke J, Lorenz A, Schwuchow A, Meyer-Zedler T, Schmitt M, Messerschmidt B, Popp J (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 10
Article Number: 207
Journal Issue: 1
DOI: 10.1038/s41377-021-00648-w
Abstract
Multimodal non-linear microscopy combining coherent anti-Stokes Raman scattering, second harmonic generation, and two-photon excited fluorescence has proved to be a versatile and powerful tool enabling the label-free investigation of tissue structure, molecular composition, and correlation with function and disease status. For a routine medical application, the implementation of this approach into an in vivo imaging endoscope is required. However, this is a difficult task due to the requirements of a multicolour ultrashort laser delivery from a compact and robust laser source through a fiber with low losses and temporal synchronization, the efficient signal collection in epi-direction, the need for small-diameter but highly corrected endomicroobjectives of high numerical aperture and compact scanners. Here, we introduce an ultra-compact fiber-scanning endoscope platform for multimodal non-linear endomicroscopy in combination with a compact four-wave mixing based fiber laser. The heart of this fiber-scanning endoscope is an in-house custom-designed, single mode, double clad, double core pure silica fiber in combination with a 2.4 mm diameter NIR-dual-waveband corrected endomicroscopic objective of 0.55 numerical aperture and 180 µm field of view for non-linear imaging, allowing a background free, low-loss, high peak power laser delivery, and an efficient signal collection in backward direction. A linear diffractive optical grating overlays pump and Stokes laser foci across the full field of view, such that diffraction-limited performance is demonstrated for tissue imaging at one frame per second with sub-micron spatial resolution and at a high transmission of 65% from the laser to the specimen using a distal resonant fiber scanner.
Involved external institutions
GRINTECH GmbH
Germany (DE)
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
Friedrich-Schiller-Universität Jena
Germany (DE)
Germany (DE)
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
Friedrich-Schiller-Universität Jena
Germany (DE)
How to cite
APA:
Pshenay-Severin, E., Bae, H., Reichwald, K., Matz, G., Bierlich, J., Kobelke, J.,... Popp, J. (2021). Multimodal nonlinear endomicroscopic imaging probe using a double-core double-clad fiber and focus-combining micro-optical concept. Light: Science & Applications, 10(1). https://doi.org/10.1038/s41377-021-00648-w
MLA:
Pshenay-Severin, Ekaterina, et al. "Multimodal nonlinear endomicroscopic imaging probe using a double-core double-clad fiber and focus-combining micro-optical concept." Light: Science & Applications 10.1 (2021).
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