Axial localization and tracking of self-interference nanoparticles by lateral point spread functions
Liu Y, Zhou Z, Wang F, Kewes G, Wen S, Burger S, Wakiani ME, Xi P, Yang J, Yang X, Benson O, Jin D (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 12
Article Number: 2019
Journal Issue: 1
DOI: 10.1038/s41467-021-22283-0
Abstract
Sub-diffraction limited localization of fluorescent emitters is a key goal of microscopy imaging. Here, we report that single upconversion nanoparticles, containing multiple emission centres with random orientations, can generate a series of unique, bright and position-sensitive patterns in the spatial domain when placed on top of a mirror. Supported by our numerical simulation, we attribute this effect to the sum of each single emitter’s interference with its own mirror image. As a result, this configuration generates a series of sophisticated far-field point spread functions (PSFs), e.g. in Gaussian, doughnut and archery target shapes, strongly dependent on the phase difference between the emitter and its image. In this way, the axial locations of nanoparticles are transferred into far-field patterns. We demonstrate a real-time distance sensing technology with a localization accuracy of 2.8 nm, according to the atomic force microscope (AFM) characterization values, smaller than 1/350 of the excitation wavelength.
Involved external institutions
University of Technology Sydney (UTS)
Australia (AU)
Humboldt-Universität zu Berlin
Germany (DE)
JCMwave
Germany (DE)
Peking University (PKU) / 北京大学
China (CN)
Australia (AU)
Humboldt-Universität zu Berlin
Germany (DE)
JCMwave
Germany (DE)
Peking University (PKU) / 北京大学
China (CN)
How to cite
APA:
Liu, Y., Zhou, Z., Wang, F., Kewes, G., Wen, S., Burger, S.,... Jin, D. (2021). Axial localization and tracking of self-interference nanoparticles by lateral point spread functions. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-22283-0
MLA:
Liu, Yongtao, et al. "Axial localization and tracking of self-interference nanoparticles by lateral point spread functions." Nature Communications 12.1 (2021).
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