Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view

Lai C, Messerschmidt B, Flaemig S, Reichwald K, Bae H, Calvarese M, Meyer T, Schmitt M, Hoffmann F, Guntinas-Lichius O, Popp J (2022)

Publication Type: Conference contribution

Publication year: 2022

Journal

Proceedings of SPIE Society of Photo-optical Instrumentation Engineers (SPIE)

Publisher: SPIE

Book Volume: 12144

Conference Proceedings Title: Proceedings of SPIE - The International Society for Optical Engineering

Event location: Virtual, Online

ISBN: 9781510651647

DOI: 10.1117/12.2621692

Abstract

Here, we report a new handheld endoscopic system for nonlinear multimodal imaging of the head and neck region. It has a long rigid endomicroscopic probe with two versions (0° and 45° bended tip), connecting with a compact scan-head of approx. 10×12×6 cm3 size. The rigid probe is 6 mm in diameter and 24 cm long and allows diffraction-limited multiphoton imaging of tissue with at least 430 µm field of view and sub-micron resolution. The signals of Coherent anti-Stokes Raman Scattering (CARS), second harmonic generation (SHG), and two-photon excited fluorescence (TPEF) are collected by a non-descanned detection path in the scan-head, and the fluorescence of Indocyanine green (ICG) labeled lesions is detected by a confocal descanned configuration. Furthermore, this system is capable of guiding high-power femtosecond laser pulses for tissue ablation without the risk of damaging optical glass components.

Involved external institutions

GRINTECH GmbH DE Germany (DE) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Friedrich-Schiller-Universität Jena DE Germany (DE) Universitätsklinikum Jena DE Germany (DE)

How to cite

APA:

Lai, C., Messerschmidt, B., Flaemig, S., Reichwald, K., Bae, H., Calvarese, M.,... Popp, J. (2022). Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view. In Jurgen Popp, Csilla Gergely (Eds.), Proceedings of SPIE - The International Society for Optical Engineering. Virtual, Online: SPIE.

MLA:

Lai, Chenting, et al. "Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view." Proceedings of the Biomedical Spectroscopy, Microscopy, and Imaging II 2022, Virtual, Online Ed. Jurgen Popp, Csilla Gergely, SPIE, 2022.

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