Label-free differentiation of clinical E. coli and Klebsiella isolates with Raman spectroscopy

Nakar A, Pistiki A, Ryabchykov O, Bocklitz T, Roesch P, Popp J (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Journal of Biophotonics Wiley-VCH Verlag

Book Volume: 15

Article Number: e202200005

Journal Issue: 7

DOI: 10.1002/jbio.202200005

Abstract

Raman spectroscopy is a promising spectroscopic technique for microbiological diagnostics. In routine diagnostic, the differentiation of pathogens of the Enterobacteriaceae family remain challenging. In this study, Raman spectroscopy was applied for the differentiation of 24 clinical E. coli, Klebsiella pneumoniae and Klebsiella oxytoca isolates. Spectra were collected with two spectroscopic approaches: UV-Resonance Raman spectroscopy (UVRR) and single-cell Raman microspectroscopy with 532 nm excitation. A description of the different biochemical profiles provided by the different excitation wavelengths was performed followed by machine-learning models for the classification at the genus and species levels. UVRR was shown to outperform 532 nm excitation, enabling correct classification at the genus level of 23/24 isolates. Furthermore, for the first time, Klebsiella species were correctly classified at the species level with 92% accuracy, classifying all three K. oxytoca isolates correctly. These findings should guide future applicative studies, increasing the scope of Raman spectroscopy's suitability for clinical applications.

Involved external institutions

Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Friedrich-Schiller-Universität Jena DE Germany (DE)

How to cite

APA:

Nakar, A., Pistiki, A., Ryabchykov, O., Bocklitz, T., Roesch, P., & Popp, J. (2022). Label-free differentiation of clinical E. coli and Klebsiella isolates with Raman spectroscopy. Journal of Biophotonics, 15(7). https://doi.org/10.1002/jbio.202200005

MLA:

Nakar, Amir, et al. "Label-free differentiation of clinical E. coli and Klebsiella isolates with Raman spectroscopy." Journal of Biophotonics 15.7 (2022).

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