Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy
Salehi H, Ramoji A, Mougari S, Merida P, Neyret A, Popp J, Horvat B, Muriaux D, Cuisinier F (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 5
Article Number: 85
Journal Issue: 1
DOI: 10.1038/s42004-022-00702-7
Abstract
SARS-CoV-2 infection remains spread worldwide and requires a better understanding of virus-host interactions. Here, we analyzed biochemical modifications due to SARS-CoV-2 infection in cells by confocal Raman microscopy. Obtained results were compared with the infection with another RNA virus, the measles virus. Our results have demonstrated a virus-specific Raman molecular signature, reflecting intracellular modification during each infection. Advanced data analysis has been used to distinguish non-infected versus infected cells for two RNA viruses. Further, classification between non-infected and SARS-CoV-2 and measles virus-infected cells yielded an accuracy of 98.9 and 97.2 respectively, with a significant increase of the essential amino-acid tryptophan in SARS-CoV-2-infected cells. These results present proof of concept for the application of Raman spectroscopy to study virus-host interaction and to identify factors that contribute to the efficient SARS-CoV-2 infection and may thus provide novel insights on viral pathogenesis, targets of therapeutic intervention and development of new COVID-19 biomarkers.
Involved external institutions
University of Montpellier / Université Montpellier
France (FR)
Friedrich-Schiller-Universität Jena
Germany (DE)
Université Claude Bernard Lyon 1 (UCB)
France (FR)
France (FR)
Friedrich-Schiller-Universität Jena
Germany (DE)
Université Claude Bernard Lyon 1 (UCB)
France (FR)
How to cite
APA:
Salehi, H., Ramoji, A., Mougari, S., Merida, P., Neyret, A., Popp, J.,... Cuisinier, F. (2022). Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy. Communications Chemistry, 5(1). https://doi.org/10.1038/s42004-022-00702-7
MLA:
Salehi, Hamideh, et al. "Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy." Communications Chemistry 5.1 (2022).
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