Using the sleeve technique in a mouse model of aortic transplantation - An instructional video
Rowinska Z, Gorressen S, Merx MW, Koeppel TA, Zernecke A, Liehn EA (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 2017
Article Number: e54915
Journal Issue: 128
DOI: 10.3791/54915
Abstract
Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the aorta in mice using the sleeve method was short and easy averaging 20 min, permitting studies of iso/allo grafts. The following article describes the aortic transplantation procedure used in our laboratory. The mice were anesthetized with a mixture of 1.5% volume isoflurane and 100% oxygen through a face mask. At this point, the segment of the aorta between the renal arteries and its bifurcation was separated from the vena cava, freely prepared and clampedat the proximal and distal segments with a single silk suture. Prior to the removal of the aorta, a saline solution containing heparin was injected into the inferior vena cava. Then the aorta was cut between the clamps and a saline heparin solution was used to flush the lumen. The sleeve technique with monofilament sutures was used in order to transplant the abdominal aorta in the orthotopic position.
Involved external institutions
Ruhr-Universität Bochum (RUB)
Germany (DE)
Universitätsklinikum Düsseldorf
Germany (DE)
KRH Klinikum Robert Koch Gehrden
Germany (DE)
Asklepios Kliniken
Germany (DE)
Julius-Maximilians-Universität Würzburg
Germany (DE)
Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen
Germany (DE)
Germany (DE)
Universitätsklinikum Düsseldorf
Germany (DE)
KRH Klinikum Robert Koch Gehrden
Germany (DE)
Asklepios Kliniken
Germany (DE)
Julius-Maximilians-Universität Würzburg
Germany (DE)
Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen
Germany (DE)
How to cite
APA:
Rowinska, Z., Gorressen, S., Merx, M.W., Koeppel, T.A., Zernecke, A., & Liehn, E.A. (2017). Using the sleeve technique in a mouse model of aortic transplantation - An instructional video. Journal of Visualized Experiments, 2017(128). https://dx.doi.org/10.3791/54915
MLA:
Rowinska, Zuzanna, et al. "Using the sleeve technique in a mouse model of aortic transplantation - An instructional video." Journal of Visualized Experiments 2017.128 (2017).
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