Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification

Roovers EF, Kaaij LJT, Redl S, Bronkhorst AW, Wiebrands K, De Jesus Domingues AM, Huang HY, Han CT, Riemer S, Dosch R, Salvenmoser W, Gruen D, Butter F, Van Oudenaarden A, Ketting RF (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Developmental Cell Elsevier (Cell Press)

Book Volume: 46

Pages Range: 285-301.e9

Journal Issue: 3

DOI: 10.1016/j.devcel.2018.07.009

Abstract

Phase separation represents an important form of subcellular compartmentalization. However, relatively little is known about how the formation or disassembly of such compartments is regulated. In zebrafish, the Balbiani body (Bb) and the germ plasm (Gp) are intimately linked phase-separated structures essential for germ cell specification and home to many germ cell-specific mRNAs and proteins. Throughout development, these structures occur as a single large aggregate (Bb), which disperses throughout oogenesis and upon fertilization accumulates again into relatively large assemblies (Gp). Formation of the Bb requires Bucky ball (Buc), a protein with prion-like properties. We found that the multi-tudor domain-containing protein Tdrd6a interacts with Buc, affecting its mobility and aggregation properties. Importantly, lack of this regulatory interaction leads to significant defects in germ cell development. Our work presents insights into how prion-like protein aggregations can be regulated and highlights the biological relevance of such regulatory events. Zebrafish Balbiani body and germ plasm are related phase-separated structures. Roovers, Kaaij et al. show that Tdrd6a is required for their formation and mobility through interaction with dimethylated arginines in the prion-like protein Bucky ball, revealing a role for Tudor domain-methylated arginine interactions in in vivo phase separation modulation.

Involved external institutions

Institut für Molekulare Biologie gGmbH / Institute of Molecular Biology (IMB) DE Germany (DE) Royal Netherlands Academy of Arts and Sciences / Koninklijke Nederlandse Akademie van Wetenschappen (KNAW) NL Netherlands (NL) Georg-August-Universität Göttingen DE Germany (DE) Leopold-Franzens-Universität Innsbruck / University of Innsbruck AT Austria (AT)

How to cite

APA:

Roovers, E.F., Kaaij, L.J.T., Redl, S., Bronkhorst, A.W., Wiebrands, K., De Jesus Domingues, A.M.,... Ketting, R.F. (2018). Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification. Developmental Cell, 46(3), 285-301.e9. https://dx.doi.org/10.1016/j.devcel.2018.07.009

MLA:

Roovers, Elke F., et al. "Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification." Developmental Cell 46.3 (2018): 285-301.e9.

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